Anti-KLH antibody (Alkaline Phosphatase) (ab34770)

Overview

  • Product name
    Anti-KLH antibody (Alkaline Phosphatase)
    See all KLH primary antibodies
  • Description
    Rabbit polyclonal to KLH (Alkaline Phosphatase)
  • Conjugation
    Alkaline Phosphatase
  • Specificity
    ab34770 recognises KLH (Keyhole Limpet Hemocyanin).
  • Tested applications
    Suitable for: Dot blot, WB, ELISAmore details
  • Species reactivity
    Reacts with: Cow
  • Immunogen

    KLH (Keyhole Limpet Hemocyanin) from Calf Intestine.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 8.00
    Preservative: 0.01% Sodium azide
    Constituents: 1% BSA, 50% Glycerol, 0.87% Sodium chloride, 0.0095% Magnesium chloride, 0.00136% Zinc chloride, 0.79% Tris HCl
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using KLH coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab34770 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot
WB
ELISA
  • Application notes
    Dot: Use at an assay dependent dilution.
    ELISA: 1/3000 - 1/13500.
    WB: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      KLH (Keyhole Limpet Hemocyanin) is a copper containing oxygen carrier occurring freely dissolved in the hemolymph of many molluscs and arthropods. KLH is often used in molecular immunology as a carrier protein conjugated to low molecular weight molecules such as peptides, amino acids, nucleic acids, drugs or toxins to render them more immunogenic due to the size of the conjugate complex and the immunogenicity of KLH. KLH forms a large complex composed of ~50 kDa subunits.
    • Cellular localization
      Secreted protein; extracellular space
    • Alternative names
      • Hemocyanin 2 c chain antibody
      • Hemocyanin 2c chain antibody
      • Hemocyanin A chain antibody
      • Hemocyanin B chain antibody
      • Keyhole limpet hemocyanin antibody
      • KLH A antibody
      • KLH B antibody
      • KLH2 c antibody
      • KLH2c antibody
      • KLHA antibody
      • KLHB antibody
      see all

    References

    ab34770 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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