Overview

  • Product nameAnti-KLH antibody (Biotin)
    See all KLH primary antibodies
  • Description
    Rabbit polyclonal to KLH (Biotin)
  • ConjugationBiotin
  • Tested applicationsSuitable for: Immunomicroscopy, ELISA, WBmore details
  • Immunogen

    KLH (Keyhole Limpet Hemocyanin)

  • General notes

    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per rabbit IgG molecule.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using KLH coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab34777 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Immunomicroscopy Use at an assay dependent dilution.
ELISA 1/20000 - 1/80000.
WB Use at an assay dependent dilution. Predicted molecular weight: 48 kDa.

Target

  • RelevanceKLH (Keyhole Limpet Hemocyanin) is a copper containing oxygen carrier occurring freely dissolved in the hemolymph of many molluscs and arthropods. KLH is often used in molecular immunology as a carrier protein conjugated to low molecular weight molecules such as peptides, amino acids, nucleic acids, drugs or toxins to render them more immunogenic due to the size of the conjugate complex and the immunogenicity of KLH. KLH forms a large complex composed of ~50 kDa subunits.
  • Cellular localizationSecreted protein; extracellular space
  • Alternative names
    • Hemocyanin 2 c chain antibody
    • Hemocyanin 2c chain antibody
    • Hemocyanin A chain antibody
    • Hemocyanin B chain antibody
    • Keyhole limpet hemocyanin antibody
    • KLH A antibody
    • KLH B antibody
    • KLH2 c antibody
    • KLH2c antibody
    • KLHA antibody
    • KLHB antibody
    see all

References for Anti-KLH antibody (Biotin) (ab34777)

ab34777 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"