Overview

  • Product name
    Anti-KLH antibody (Biotin)
    See all KLH primary antibodies
  • Description
    Rabbit polyclonal to KLH (Biotin)
  • Conjugation
    Biotin
  • Tested applications
    Suitable for: Immunomicroscopy, ELISA, WBmore details
  • Immunogen

    KLH (Keyhole Limpet Hemocyanin)

  • General notes

    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per rabbit IgG molecule.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using KLH coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab34777 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Immunomicroscopy Use at an assay dependent dilution.
ELISA 1/20000 - 1/80000.
WB Use at an assay dependent dilution. Predicted molecular weight: 48 kDa.

Target

  • Relevance
    KLH (Keyhole Limpet Hemocyanin) is a copper containing oxygen carrier occurring freely dissolved in the hemolymph of many molluscs and arthropods. KLH is often used in molecular immunology as a carrier protein conjugated to low molecular weight molecules such as peptides, amino acids, nucleic acids, drugs or toxins to render them more immunogenic due to the size of the conjugate complex and the immunogenicity of KLH. KLH forms a large complex composed of ~50 kDa subunits.
  • Cellular localization
    Secreted protein; extracellular space
  • Alternative names
    • Hemocyanin 2 c chain antibody
    • Hemocyanin 2c chain antibody
    • Hemocyanin A chain antibody
    • Hemocyanin B chain antibody
    • Keyhole limpet hemocyanin antibody
    • KLH A antibody
    • KLH B antibody
    • KLH2 c antibody
    • KLH2c antibody
    • KLHA antibody
    • KLHB antibody
    see all

References

ab34777 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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