Anti-KLH antibody (FITC) (ab34766)

Overview

  • Product nameAnti-KLH antibody (FITC)
    See all KLH primary antibodies
  • Description
    Rabbit polyclonal to KLH (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • Tested applicationsSuitable for: Immunomicroscopy, Flow Cytmore details
  • Immunogen

    Full length protein KLH (Keyhole Limpet Hemocyanin)

  • General notes

    3.4 moles FITC per mole of rabbit IgG.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 0.15% Sodium chloride, 10mg/ml BSA, 0.02M Potassium phosphate. pH 7.2
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab34766 was prepared from monospecific antiserum by immunoaffinity chromatography using KLH coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab34766 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Immunomicroscopy
Flow Cyt
  • Application notesFlow Cyt: Use at an assay dependent dilution.
    IM: Use at an assay dependent dilution.
    Other antibody based fluorescent assays requiring lot-to-lot consistency: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceKLH (Keyhole Limpet Hemocyanin) is a copper containing oxygen carrier occurring freely dissolved in the hemolymph of many molluscs and arthropods. KLH is often used in molecular immunology as a carrier protein conjugated to low molecular weight molecules such as peptides, amino acids, nucleic acids, drugs or toxins to render them more immunogenic due to the size of the conjugate complex and the immunogenicity of KLH. KLH forms a large complex composed of ~50 kDa subunits.
    • Cellular localizationSecreted protein; extracellular space
    • Alternative names
      • Hemocyanin 2 c chain antibody
      • Hemocyanin 2c chain antibody
      • Hemocyanin A chain antibody
      • Hemocyanin B chain antibody
      • Keyhole limpet hemocyanin antibody
      • KLH A antibody
      • KLH B antibody
      • KLH2 c antibody
      • KLH2c antibody
      • KLHA antibody
      • KLHB antibody
      see all

    References for Anti-KLH antibody (FITC) (ab34766)

    ab34766 has not yet been referenced specifically in any publications.

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    Yes, ab47052 would bind to F(ab')2 since this antibody reacts with the heavy chains on Mouse IgG and with light chains common to most mouse immunoglobulins. The F(ab')2 fragment contains the light chains and part...

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