Anti-KLH antibody (TRITC) (ab34706)

Overview

  • Product name
    Anti-KLH antibody (TRITC)
    See all KLH primary antibodies
  • Description
    Rabbit polyclonal to KLH (TRITC)
  • Conjugation
    TRITC. Ex: 547nm, Em: 572nm
  • Tested applications
    Suitable for: Flow Cyt, ICC/IF, Immunomicroscopymore details
  • Species reactivity
    Reacts with Keyhole limpet. Not yet tested in other species.
  • Immunogen

    Full length protein -KLH.

  • General notes

    Tetramethylrhodamine isothiocyanate (TRITC)(Molecular Weight 444 daltons) Absorption Wavelength: 550 nm, Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 2.8 moles TRITC per mole of Rabbit IgG.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using KLH coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against KLH.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab34706 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/2000 - 1/10000.
ICC/IF Use at an assay dependent dilution.
Immunomicroscopy 1/500 - 1/2500.

Target

  • Relevance
    KLH (Keyhole Limpet Hemocyanin) is a copper containing oxygen carrier occurring freely dissolved in the hemolymph of many molluscs and arthropods. KLH is often used in molecular immunology as a carrier protein conjugated to low molecular weight molecules such as peptides, amino acids, nucleic acids, drugs or toxins to render them more immunogenic due to the size of the conjugate complex and the immunogenicity of KLH. KLH forms a large complex composed of ~50 kDa subunits.
  • Cellular localization
    Secreted protein; extracellular space
  • Alternative names
    • Hemocyanin 2 c chain antibody
    • Hemocyanin 2c chain antibody
    • Hemocyanin A chain antibody
    • Hemocyanin B chain antibody
    • Keyhole limpet hemocyanin antibody
    • KLH A antibody
    • KLH B antibody
    • KLH2 c antibody
    • KLH2c antibody
    • KLHA antibody
    • KLHB antibody
    see all

References for Anti-KLH antibody (TRITC) (ab34706)

ab34706 has not yet been referenced specifically in any publications.

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