Overview

  • Product nameAnti-KLHL14 antibody
    See all KLHL14 primary antibodies
  • Description
    Rabbit polyclonal to KLHL14
  • SpecificityThis antibody recognizes KLHL14
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    A region within synthetic peptide: MSRSGDRTST FDPSHSDNLL HGLNLLWRKQ LFCDVTLTAQ GQQFHCHKAV, corresponding to N terminal amino acids 1-50 of Human KLHL14

  • Positive control
    • HepG2 cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab49353 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB Use a concentration of 5 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 43 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Anti-KLHL14 antibody images

  • Anti-KLHL14 antibody (ab49353) at 5 µg/ml + HepG2 cell lysate at 10 µg with 5% skim milk in PBS.

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 43 kDa
    Observed band size : 39 kDa (why is the actual band size different from the predicted?)

References for Anti-KLHL14 antibody (ab49353)

ab49353 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"