Overview

  • Product name
  • Description
    Rabbit polyclonal to KLHL18
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: EAKDYHLMPE RRPHLPAFRT RPRCCTSIAG LIYAVGGLNS AGDSLNVVEV FDPIANCWER CRPMTTARSR VGVAVVNGL, corresponding to amino acids 259-337 of Human KLHL18.

  • Positive control
    • Human heart muscle tissue.
  • General notes


    ab122243 is mono-specific.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Primary antibody notes
    ab122243 is mono-specific.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122243 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

  • Sequence similarities
    Contains 1 BACK (BTB/Kelch associated) domain.
    Contains 1 BTB (POZ) domain.
    Contains 6 Kelch repeats.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ13703 antibody
    • Kelch like 18 (Drosophila) antibody
    • Kelch like 18 antibody
    • Kelch-like protein 18 antibody
    • KIAA0795 antibody
    • KLH18_HUMAN antibody
    • KLHL 18 antibody
    • KLHL18 antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm. Recommended concentration of ab122243 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122243, at 1/50 dilution, staining KLHL18 in paraffin-embedded Human heart muscle tissue by Immunohistochemistry.

References

ab122243 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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