Overview

  • Product nameAnti-KLHL21 antibody
  • Description
    Rabbit polyclonal to KLHL21
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 345-520 of Human KLHL21

  • Positive control
    • WB: A549, HeLa, Jurkat and Raji whole cell lysates IHC-P: Hepatoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab111604 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 67 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with 10mM citrate buffer pH 6.0 or Tris-EDTA buffer pH 8.0 before commening with IHC staining protocol.

Target

  • FunctionSubstrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex required for efficient chromosome alignment and cytokinesis. The BCR(KLHL21) E3 ubiquitin ligase complex regulates localization of the chromosomal passenger complex (CPC) from chromosomes to the spindle midzone in anaphase and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesContains 1 BACK (BTB/Kelch associated) domain.
    Contains 1 BTB (POZ) domain.
    Contains 6 Kelch repeats.
  • Cellular localizationCytoplasm > cytoskeleton > spindle. Localizes to the spindle midzone and targets CUL3 to this region.
  • Information by UniProt
  • Database links
  • Alternative names
    • Kelch-like protein 21 antibody
    • KIAA0469 antibody
    • KLH21_HUMAN antibody
    • KLHL21 antibody
    • MGC99635 antibody
    see all

Anti-KLHL21 antibody images

  • Anti-KLHL21 antibody (ab111604) at 1/1000 dilution + A549 whole cell lysate at 30 µg

    Predicted band size : 67 kDa
  • ab111604, at a 1/500, staining KLHL21 in paraffin-embedded Hepatoma tissue.

References for Anti-KLHL21 antibody (ab111604)

ab111604 has not yet been referenced specifically in any publications.

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