Overview

  • Product name
  • Description
    Rabbit polyclonal to KLHL26
  • Specificity
    This antibody recognizes KLHL26
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Zebrafish
  • Immunogen

    A region within synthetic peptide: YNWRLNNVTG IVQVYNTDTD EWERDLHFPE SFAGIACAPV LLPRAGTR, corresponding to C terminal amino acids 567-616 of Human KLHL26

  • Positive control
    • Jurkat cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab48972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 68 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Anti-KLHL26 antibody images

  • Lane 1 : MARKER
    Lane 2 : Anti-KLHL26 antibody (ab48972) at 1.25 µg/ml

    Lane 1 : As above
    Lane 2 : Jurkat cell lysate at 10 µg with 5% skimmed milk in PBS.

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 68 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)

References for Anti-KLHL26 antibody (ab48972)

ab48972 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rabbit Tissue sections (Stomach)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
Yes - H2O2
Specification
Stomach
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde
Username

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Submitted Mar 28 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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