Overview

  • Product name
  • Description
    Rabbit polyclonal to KLHL3
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 1 and 306 of Human KLHL3 (Uniprot ID: Q9UH77)

  • Positive control
    • A549, HeLa, HepG2, HCT116

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab153838 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Predicted molecular weight: 65 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used.

Target

  • Relevance
    KLHL3 protein contains a poxvirus and zinc finger domain at the N-terminus and six tandem repeats (kelch repeats) at the C-terminus. At the amino acid level, KLHL3 shares 77% similarity with Drosophila kelch and 89% similarity with Mayven (KLHL2), another human kelch homolog. At least three isoforms are produced and may be the result of alternative promoter usage. The KLHL3 maps within the smallest commonly deleted segment in myeloid leukemias characterized by a deletion of 5q; however, no inactivating mutations of KLHL3 could be detected in malignant myeloid disorders with loss of 5q.
  • Cellular localization
    Cytoplasmic and cytoskeleton.
  • Database links
  • Alternative names
    • FLJ40871 antibody
    • kelch (Drosophila) like 3 antibody
    • kelch like 3 (Drosophila) antibody
    • kelch like 3 antibody
    • Kelch like protein 3 antibody
    • KIAA1129 antibody
    • KLHL 3 antibody
    • KLHL3 antibody
    • MGC44594 antibody
    see all

Images

  • Anti-KLHL3 antibody (ab153838) at 1/10000 dilution + A549 whole cell lysate at 30 µg

    Predicted band size : 65 kDa
  • Immunohistochemical analysis of paraffin-embedded U87 xenograft using ab153838 antibody at 1/500 dilution.

References

ab153838 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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