Overview

  • Product name
  • Description
    Rabbit polyclonal to KLHL3
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Rabbit, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide: EGESVKLSSQ TLIQAGDDEK NQRTITVNPA HMGKAFKVMN ELRSKQLLCD , corresponding to amino acids 2-51 of Human KLHL3

  • Positive control
    • HepG2 cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab66655 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 65 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Relevance
    KLHL3 protein contains a poxvirus and zinc finger domain at the N-terminus and six tandem repeats (kelch repeats) at the C-terminus. At the amino acid level, KLHL3 shares 77% similarity with Drosophila kelch and 89% similarity with Mayven (KLHL2), another human kelch homolog. At least three isoforms are produced and may be the result of alternative promoter usage. The KLHL3 maps within the smallest commonly deleted segment in myeloid leukemias characterized by a deletion of 5q; however, no inactivating mutations of KLHL3 could be detected in malignant myeloid disorders with loss of 5q.
  • Cellular localization
    Cytoplasmic and cytoskeleton.
  • Database links
  • Alternative names
    • FLJ40871 antibody
    • kelch (Drosophila) like 3 antibody
    • kelch like 3 (Drosophila) antibody
    • kelch like 3 antibody
    • Kelch like protein 3 antibody
    • KIAA1129 antibody
    • KLHL 3 antibody
    • KLHL3 antibody
    • MGC44594 antibody
    see all

Images

  • Anti-KLHL3 antibody (ab66655) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 65 kDa
    Observed band size : >70 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Roy A  et al. Generation of WNK1 knockout cell lines by CRISPR/Cas-mediated genome editing. Am J Physiol Renal Physiol 308:F366-76 (2015). Read more (PubMed: 25477473) »
  • Gong Y  et al. KLHL3 regulates paracellular chloride transport in the kidney by ubiquitination of claudin-8. Proc Natl Acad Sci U S A 112:4340-5 (2015). Read more (PubMed: 25831548) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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