Overview

  • Product nameAnti-KLHL3 antibody
    See all KLHL3 primary antibodies
  • Description
    Rabbit polyclonal to KLHL3
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide from within residues: YVVGGDDGSC NLASVEYYNP VTDKWTLLPT NMSTGRSYAG VAVIHKSL, corresponding to internal sequence amino acids 540-587 of Human KLHL3 (NP_059111)

  • Positive control
    • HepG2 cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab86390 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 65 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • RelevanceKLHL3 protein contains a poxvirus and zinc finger domain at the N-terminus and six tandem repeats (kelch repeats) at the C-terminus. At the amino acid level, KLHL3 shares 77% similarity with Drosophila kelch and 89% similarity with Mayven (KLHL2), another human kelch homolog. At least three isoforms are produced and may be the result of alternative promoter usage. The KLHL3 maps within the smallest commonly deleted segment in myeloid leukemias characterized by a deletion of 5q; however, no inactivating mutations of KLHL3 could be detected in malignant myeloid disorders with loss of 5q.
  • Cellular localizationCytoplasmic and cytoskeleton.
  • Database links
  • Alternative names
    • FLJ40871 antibody
    • kelch (Drosophila) like 3 antibody
    • kelch like 3 (Drosophila) antibody
    • kelch like 3 antibody
    • Kelch like protein 3 antibody
    • KIAA1129 antibody
    • KLHL 3 antibody
    • KLHL3 antibody
    • MGC44594 antibody
    see all

Anti-KLHL3 antibody images

  • Anti-KLHL3 antibody (ab86390) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 65 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 42 kDa. We are unsure as to the identity of these extra bands.

References for Anti-KLHL3 antibody (ab86390)

This product has been referenced in:
  • Louis-Dit-Picard H  et al. KLHL3 mutations cause familial hyperkalemic hypertension by impairing ion transport in the distal nephron. Nat Genet : (2012). Read more (PubMed: 22406640) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab86390.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"