• Product name
  • Description
    Rabbit polyclonal to Klotho
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide of 17 amino acids (Mouse) (C terminal).



Our Abpromise guarantee covers the use of ab18131 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 116 kDa.
ELISA 1/10000 - 1/100000.


  • Function
    May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D (By similarity). Essential factor for the specific interaction between FGF23 and FGFR1.
    The Klotho peptide generated by cleavage of the membrane-bound isoform may be an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling.
  • Tissue specificity
    Present in cortical renal tubules (at protein level). Soluble peptide is present in serum and cerebrospinal fluid. Expressed in kidney, placenta, small intestine and prostate. Down-regulated in renal cell carcinomas, hepatocellular carcinomas, and in chronic renal failure kidney.
  • Involvement in disease
    Defects in KL are a cause of hyperphosphatemic familial tumoral calcinosis (HFTC) [MIM:211900]. A severe metabolic disorder that manifests with hyperphosphatemia and massive calcium deposits in the skin and subcutaneous tissues. Some patients manifest recurrent, transient, painful swellings of the long bones associated with the radiographic findings of periosteal reaction and cortical hyperostosis and absence of skin involvement.
  • Sequence similarities
    Belongs to the glycosyl hydrolase 1 family. Klotho subfamily.
  • Domain
    Contains 2 glycosyl hydrolase 1 regions. However, the first region lacks the essential Glu active site residue at position 239, and the second one lacks the essential Glu active site residue at position 872.
  • Post-translational
  • Cellular localization
    Secreted and Cell membrane. Isoform 1 shedding leads to a soluble peptide.
  • Information by UniProt
  • Database links
  • Alternative names
    • Kl antibody
    • KLOT_HUMAN antibody
    • Klotho peptide antibody

References for Anti-Klotho antibody (ab18131)

This product has been referenced in:
  • Sun H  et al. Overexpression of Klotho suppresses liver cancer progression and induces cell apoptosis by negatively regulating wnt/ß-catenin signaling pathway. World J Surg Oncol 13:307 (2015). WB . Read more (PubMed: 26499380) »
  • Labrie V  et al. Serine racemase is associated with schizophrenia susceptibility in humans and in a mouse model. Hum Mol Genet 18:3227-43 (2009). WB ; Mouse . Read more (PubMed: 19483194) »

See all 3 Publications for this product

Product Wall

Western blot
Rat Tissue lysate - whole (Hippocampus)
Loading amount
30 µg
Gel Running Conditions
Non-reduced Denaturing (14% gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Mr. Gareth Bowen

Verified customer

Submitted Apr 25 2013

Thank you very much for your interest in ab18131.
To our knowledge, ab18131 has not been tested in Human. Therefore, I can offer a discount off a future purchase if you buy ab18131 now, test it in Human and submit feedback to us in the form of an ...

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Thank you for your inquiry and I apologize for the delay in this response. The C-terminal immunizing peptide used to generate ab18131 is based on the 1014 amino acid sequence of membrane-bound Klotho. This antibody should only recognize the membr...

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Abcam has not validated the combination of species/application used in this Abreview.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Mouse Tissue sections (choroid plexus, kidney)
choroid plexus, kidney
Antigen retrieval step
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Jan 27 2006

Thank you for your enquiry. The antibody orignates outside of Abcam and the originator of the antibody never reveals an immunogen peptide sequence. All I can provide is: "17aa peptide sequence near the C-terminus of mouse Klotho." I hope this inf...

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Thank you for your email. We do not carry klotho recombinant protein. I did a Google search, but unfortunately couldn't see any companies that do sell it. I'm still looking into your original question regarding ab18131 and will get back to you once...

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