Anti-KLRA3 antibody (ab84005)


  • Product name
    Anti-KLRA3 antibody
  • Description
    Rabbit polyclonal to KLRA3
  • Host species
  • Specificity
    ab84005 may cross-react with other LY49 receptors.
  • Tested applications
    Suitable for: WB, ELISA, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    A 14 amino acid synthetic peptide near the carboxy terminus of Mouse KLRA3 (AAI32525).

  • Positive control
    • Mouse brain tissue lysate.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab84005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 31 kDa.
ELISA Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.



  • Relevance
    KLRA3 (also known as Ly49C) is a member of the LY49 family of receptors in Natural Killer (NK) cells that bind to major histocompatibility complex (MHC) class 1. These proteins are classified as either activating or inhibitory receptors based on whether they possess an immunoreceptor tyrosine-based inhibitory motif (ITIM) in their cytoplasmic region (for inhibitory receptors), or an immunoreceptor tyrosine-based activation motif (ITAM) that transmits activating signals resulting in phosphorylation of several substrates. KLRA3 is thought to be an inhibitory receptor, recognizing peptide–receptive H-2Kb.
  • Cellular localization
    Membrane; Single-pass type II membrane protein.
  • Database links
  • Alternative names
    • 5E6 antibody
    • Killer cell lectin like receptor 3 antibody
    • Killer cell lectin like receptor subfamily A member 3 antibody
    • KLRA 3 antibody
    • Ly 49c antibody
    • Ly49C antibody
    • Lymphocyte antigen 49c antibody
    • MGC123910 antibody
    • Natural killer cell associated antigen 2 antibody
    • Nk 2 antibody
    • NK 2.1 antibody
    • Nk2 antibody
    • Nk2.1 antibody
    • OTTMUSP00000026267 antibody
    • T cell surface glycoprotein Ly 49C antibody
    see all


  • Anti-KLRA3 antibody (ab84005) at 2 µg/ml + Mouse brain tissue lysate at 15 µg

    Predicted band size: 31 kDa
    Observed band size: 31 kDa

  • Flow cytometry analysis of rat spleen, staining KLRA3 with ab84005.

    Red blood cells were lysed using ACK lysing buffer. The sample was incubated with the primary antibody (1/50 in PBS) for 30 minutes at 4°C. A FITC-conjugated goat anti-rabbit polyclonal IgG (1/200) was used as the secondary antibody.

    Gating Strategy: Live cells

    See Abreview


ab84005 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you very much for conatcting Abcam.

To our knowledge, ab84005 has not been tested in IHC-P and we are unable to provide free sample sizes of our products. However by participating in our AbTrial program you can now use our products in ...

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The Abreview has been published so teh discount code can be used.

If you need any further assistance in the future, please do not hesitate to contact me.

Abcam guarantees this product to work in the species/application used in this Abreview.
Flow Cytometry
Rat Cell (Spleen)
Cell harvesting/tissue preparation method: ACK lysis red cells
Sample buffer: PBS
Gating Strategy
life cells

Abcam user community

Verified customer

Submitted Jul 19 2012

Thank you for your updates.

Would you be so kind to attach the results as jpeg image to the Abreview? It would be nice to have some supporting data which would help future customers who will read this feedback. This is particularly important ...

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Thank you for your enquiry.

I have checked our system and can confirm that the Abreview has not been submitted properly - it is still in edited status. In order to validate the discount code, the Abreview should reach a certain standard (see ...

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Expiration date: [27/09/2012]

I am very pleased to hear you would like to accept our offer and testab84005 in Flow cytometry on rat samples. This code will give you: 1 freeFlow cytometry on rat samples before t...

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Thank you for your enquiry and your interest.

I can certainly offer a discount for testing ab84005 in Flow cytometry using rat samples. Could you please pass the customer's contact details so that I can issue the reference number as soon as p...

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Thank you for contacting Abcam regarding ab84005.

I am sorry that you have been experiencing difficulties with this antibody in WB. I have reviewed the protocol information you provided and would like to make some suggestions to improve your...

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