The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 1µg for 106 cells. ab18420-Syrian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.
Use at an assay dependent concentration.
FunctionPlays an inhibitory role on natural killer (NK) cells and T-cell functions upon binding to their non-MHC ligands. May mediate missing self recognition by binding to a highly conserved site on classical cadherins, enabling it to monitor expression of E-cadherin/CDH1, N-cadherin/CDH2 and R-cadherin/CDH4 on target cells.
Tissue specificityExpressed specifically on natural killer (NK) cells and T-cells, mainly CD8 T-cells.
DomainContains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. Upon phosphorylation of ITIM motif KLRG1 associates with the two phosphatases PTPN11 and INPP5D.
Mast cell function-associated antigen, rat, homolog of antibody
Anti-KLRG1 antibody [2F1] (FITC) images
Flow Cytometry - Anti-KLRG1 antibody [2F1] (FITC) (ab24867)Image from Durward M et al., PLoS One. 2012;7(4):e34925. Epub 2012 Apr 25. Fig 4.; doi:10.1371/journal.pone.0034925; April 25, 2012, PLoS ONE 7(4): e34925.
Flow cytometry analysis of splenocytes from mice, staining KLRG1 with ab24867.
References for Anti-KLRG1 antibody [2F1] (FITC) (ab24867)
This product has been referenced in:
Valkenburg SA et al. Early priming minimizes the age-related immune compromise of CD8? T cell diversity and function. PLoS Pathog8:e1002544 (2012).
Read more (PubMed: 22383879) »
Durward M et al. Active Evasion of CTL Mediated Killing and Low Quality Responding CD8+ T Cells Contribute to Persistence of Brucellosis. PLoS One7:e34925 (2012).
Read more (PubMed: 22558103) »