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Recombinant fusion protein, MBP-SUV39H1.
Our Abpromise guarantee covers the use of ab12405 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent dilution.|
|ELISA||Use at an assay dependent dilution.|
|IP||Use at an assay dependent dilution.|
|WB||Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 48 kDa. The target may be expressed at low levels and we would recommend a highly enriched nuclear extract as a sample for WB. Additionally, a signal amplification step using a biotin conjugate as a secondary antibody is preferrable over the enzyme conjugated secondary antibody method.|
|ICC||Use a concentration of 5 µg/ml. A signal amplification step using a biotin conjugate as a secondary antibody is preferrable over the enzyme conjugated secondary antibody method.|
Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 25 µg of chromatin, 8 µg of ab12405 (blue), and 20 µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.
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