![ChIP - SUV39H1 antibody [44.1] - ChIP Grade (ab12405) image](#ab12405_2.gif)
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Overview
Product name
Anti-KMT1A / SUV39H1 antibody [44.1] - ChIP Grade
See all KMT1A / SUV39H1 products (8) ...
Description
Mouse monoclonal [44.1] to KMT1A / SUV39H1 - ChIP Grade
Tested applications
ChIP, ELISA, IP, WB, ICCmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Recombinant fusion protein, MBP-SUV39H1.
Epitope
Ab 12405 recognizes an epitope in the N-terminal (195 amino acids) of human and mouse SUV39H1 Histone Methyltransferase.
Positive control
HeLa cell lysate
General notes
Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Clone number
44.1
Isotype
IgG1
Research areas
Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Cancer >> Cell cycle >> Cell cycle inhibitors >> Rb
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Methylation >> Lysine methylation
Microbiology >> Interspecies Interaction >> Host Virus Interaction
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Methylation
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ChIP - SUV39H1 antibody [44.1] - ChIP Grade (ab12405)
![ChIP - SUV39H1 antibody [44.1] - ChIP Grade (ab12405) image](/ps/datasheet/Images/12/ab12405/ab12405_2.gif)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab12405 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ChIP: Use at an assay dependent dilution.
ELISA: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use a concentration of 2 - 4 µg/ml.Predicted molecular weight: 48 kDa.(The target may be expressed at low levels and we would recommend a highly enriched nuclear extract as a sample for WB. Additionally, a signal amplification step using a biotin conjugate as a secondary antibody is preferrable over the enzyme conjugated secondary antibody method.)
ICC: Use a concentration of 5 µg/ml(A signal amplification step using a biotin conjugate as a secondary antibody is preferrable over the enzyme conjugated secondary antibody method.)
Target
Function
Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys-9' as substrate. Also weakly methylates histone H1 (in vitro). H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin at pericentric and telomere regions. H3 'Lys-9' trimethylation is also required to direct DNA methylation at pericentric repeats. SUV39H1 is targeted to histone H3 via its interaction with RB1 and is involved in many processes, such as repression of MYOD1-stimulated differentiation, regulation of the control switch for exiting the cell cycle and entering differentiation, repression by the PML-RARA fusion protein, BMP-induced repression, repression of switch recombination to IgA and regulation of telomere length. Component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD(+)/NADP(+) ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus.
Sequence similarities
Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily.
Contains 1 chromo domain.
Contains 1 post-SET domain.
Contains 1 pre-SET domain.
Contains 1 SET domain.
Developmental stage
Accumulates during mitosis at centromeres during prometaphase, but dissociates from the centromere at the meta- to anaphase transition.
Domain
Although the SET domain contains the active site of enzymatic activity, both pre-SET and post-SET domains are required for methyltransferase activity. The SET domain also participates to stable binding to heterochromatin.
Post-translational
modifications
Phosphorylated on serine residues, and to a lesser degree, on threonine residues. The phosphorylated form is stabilized by SBF1 and is less active in its transcriptional repressor function.
Acetylated at Lys-266, leading to inhibition of enzyme activity. SIRT1-mediated deacetylation relieves this inhibition.
Cellular localization
Nucleus. Chromosome > centromere. Associates with centromeric constitutive heterochromatin.
Target information above from: UniProt accessionO43463
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- H3 K9 HMTase antibody
- H3 K9 HMTase1 antibody
- H3-K9-HMTase 1 antibody
see all
Database links
- Entrez Gene: 6839 Human
- Entrez Gene: 20937 Mouse
- Entrez Gene: 302553 Rat
- Omim: 300254 Human
- SwissProt: O43463 Human
- SwissProt: O54864 Mouse
- Unigene: 37936 Human
- Unigene: 522639 Human
see all
Anti-KMT1A / SUV39H1 antibody [44.1] - ChIP Grade images:
ChIP - SUV39H1 antibody [44.1] - ChIP Grade (ab12405)
Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 25 µg of chromatin, 8 µg of ab12405 (blue), and 20 µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.
Protocols
References for Anti-KMT1A / SUV39H1 antibody [44.1] - ChIP Grade (ab12405)
This product has been referenced in:
- Wakabayashi Yet al. Histone 3 Lysine 9 (H3K9) Methyltransferase Recruitment to the Interleukin-2 (IL-2) Promoter Is a Mechanism of Suppression of IL-2 Transcription by the Transforming Growth Factor-{beta}-Smad Pathway. J Biol Chem 286:35456-65 (2011). ChIP; Mouse.Read more (PubMed: 21862595) »
- Alder Oet al. Ring1B and Suv39h1 delineate distinct chromatin states at bivalent genes during early mouse lineage commitment. Development 137:2483-92 (2010). ChIP; Mouse.Read more (PubMed: 20573702) »
See all 7 publications for this product
Publishing research using ab12405? Please let us know so that we can cite the reference in this datasheet
![SUV39H1 antibody [44.1] - ChIP Grade for ChIP in Rat (12405)](/ps/Reviews/Images/ab7310_30342.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"