Anti-KMT1A / SUV39H1 antibody (ab38637)

Overview

• Product name
  Anti-KMT1A / SUV39H1 antibody
  See all KMT1A / SUV39H1 primary antibodies
• Description
  Rabbit polyclonal to KMT1A / SUV39H1
• Tested applications
  Suitable for: ICC/IF, WB, ELISAmore details
  Unsuitable for: IHC-P
• Species reactivity
  Reacts with: Mouse, Human
  
• Immunogen

  Fusion protein corresponding to Human KMT1A/ SUV39H1 (N terminal).
  Database link: 6839

• Positive control
  • AOS-2 cell lysate, NIH/3T3 cell lysate and human breast carcinoma tissue.

Properties

• Form
  Liquid
• Storage instructions
  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
• Storage buffer
  Preservative: 0.09% Sodium Azide
  Constituents: PBS
• Concentration information loading...
• Purity
  Protein G purified
• Purification notes
  This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
• Clonality
  Polyclonal
• Isotype
  IgG
• Research areas
  • Epigenetics and Nuclear Signaling
  • Chromatin Modifying Enzymes
  • Methylation

  • Microbiology
  • Interspecies Interaction
  • Host Virus Interaction

  • Epigenetics and Nuclear Signaling
  • Chromatin Modifying Enzymes
  • Methylation
  • Lysine methylation

  • Cancer
  • Cell cycle
  • Cell cycle inhibitors
  • Rb

Associated products

• Compatible Secondaries
  • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
• Positive Controls
  • NIH 3T3 nuclear extract lysate (ab14874)

Applications

Target

• Function
  Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys-9' as substrate. Also weakly methylates histone H1 (in vitro). H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin at pericentric and telomere regions. H3 'Lys-9' trimethylation is also required to direct DNA methylation at pericentric repeats. SUV39H1 is targeted to histone H3 via its interaction with RB1 and is involved in many processes, such as repression of MYOD1-stimulated differentiation, regulation of the control switch for exiting the cell cycle and entering differentiation, repression by the PML-RARA fusion protein, BMP-induced repression, repression of switch recombination to IgA and regulation of telomere length. Component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD(+)/NADP(+) ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. Recruited by the large PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1, contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation.
• Sequence similarities
  Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar3-9 subfamily.
  Contains 1 chromo domain.
  Contains 1 post-SET domain.
  Contains 1 pre-SET domain.
  Contains 1 SET domain.
• Developmental stage
  Accumulates during mitosis at centromeres during prometaphase, but dissociates from the centromere at the meta- to anaphase transition.
• Domain
  Although the SET domain contains the active site of enzymatic activity, both pre-SET and post-SET domains are required for methyltransferase activity. The SET domain also participates to stable binding to heterochromatin.
  In the pre-SET domain, Cys residues bind 3 zinc ions that are arranged in a triangular cluster; some of these Cys residues contribute to the binding of two zinc ions within the cluster.
• Post-translational
  modifications
  Phosphorylated on serine residues, and to a lesser degree, on threonine residues. The phosphorylated form is stabilized by SBF1 and is less active in its transcriptional repressor function.
  Acetylated at Lys-266, leading to inhibition of enzyme activity. SIRT1-mediated deacetylation relieves this inhibition.
• Cellular localization
  Nucleus. Nucleus lamina. Nucleus, nucleoplasm. Chromosome, centromere. Associates with centromeric constitutive heterochromatin.

• Target information above from: UniProt accession O43463 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• Database links
  • Entrez Gene: 6839 Human
  • Entrez Gene: 20937 Mouse
  • Omim: 300254 Human
  • SwissProt: O43463 Human
  • SwissProt: O54864 Mouse
  • Unigene: 522639 Human
  • Unigene: 479743 Mouse
  • Unigene: 9244 Mouse
• Alternative names
  • H3 K9 HMTase1 antibody
  • H3-K9-HMTase 1 antibody
  • Histone H3-K9 methyltransferase 1 antibody

  • Histone H3-K9 methyltransferase1 antibody
  • Histone lysine N methyltransferase, H3 lysine 9 specific 1 antibody
  • Histone-lysine N-methyltransferase SUV39H1 antibody
  • KMT1 A antibody
  • KMT1A antibody
  • Lysine N methyltransferase 1A antibody
  • Lysine N-methyltransferase 1A antibody
  • MG44 antibody
  • mIS6 antibody
  • Position-effect variegation 3-9 homolog antibody
  • Su(var)3 9 homolog 1 antibody
  • Su(var)3-9 homolog 1 antibody
  • Suppressor of variegation 3 9 homolog 1 (Drosophila) antibody
  • Suppressor of variegation 3-9 homolog 1 antibody
  • SUV39 H1 antibody
  • SUV39H antibody
  • SUV39H1 antibody
  • SUV91_HUMAN antibody

  see all

Protocols

• Immunocytochemistry & immunofluorescence protocols
• Western blot protocols