• Product nameAnti-KMT1A / SUV39H1 antibody
    See all KMT1A / SUV39H1 primary antibodies
  • Description
    Rabbit polyclonal to KMT1A / SUV39H1
  • Tested applicationsSuitable for: ICC/IF, WB, ELISAmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Fusion protein corresponding to Human KMT1A/ SUV39H1 (N terminal).
    Database link: 6839

  • Positive control
    • AOS-2 cell lysate, NIH/3T3 cell lysate and human breast carcinoma tissue.



Our Abpromise guarantee covers the use of ab38637 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
WB 1/100 - 1/500. Predicted molecular weight: 48 kDa.
ELISA 1/1000.
  • Application notesIs unsuitable for IHC-P.
  • Target

    • FunctionHistone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys-9' as substrate. Also weakly methylates histone H1 (in vitro). H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin at pericentric and telomere regions. H3 'Lys-9' trimethylation is also required to direct DNA methylation at pericentric repeats. SUV39H1 is targeted to histone H3 via its interaction with RB1 and is involved in many processes, such as repression of MYOD1-stimulated differentiation, regulation of the control switch for exiting the cell cycle and entering differentiation, repression by the PML-RARA fusion protein, BMP-induced repression, repression of switch recombination to IgA and regulation of telomere length. Component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD(+)/NADP(+) ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. Recruited by the large PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1, contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation.
    • Sequence similaritiesBelongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar3-9 subfamily.
      Contains 1 chromo domain.
      Contains 1 post-SET domain.
      Contains 1 pre-SET domain.
      Contains 1 SET domain.
    • Developmental stageAccumulates during mitosis at centromeres during prometaphase, but dissociates from the centromere at the meta- to anaphase transition.
    • DomainAlthough the SET domain contains the active site of enzymatic activity, both pre-SET and post-SET domains are required for methyltransferase activity. The SET domain also participates to stable binding to heterochromatin.
      In the pre-SET domain, Cys residues bind 3 zinc ions that are arranged in a triangular cluster; some of these Cys residues contribute to the binding of two zinc ions within the cluster.
    • Post-translational
      Phosphorylated on serine residues, and to a lesser degree, on threonine residues. The phosphorylated form is stabilized by SBF1 and is less active in its transcriptional repressor function.
      Acetylated at Lys-266, leading to inhibition of enzyme activity. SIRT1-mediated deacetylation relieves this inhibition.
    • Cellular localizationNucleus. Nucleus lamina. Nucleus, nucleoplasm. Chromosome, centromere. Associates with centromeric constitutive heterochromatin.
    • Information by UniProt
    • Database links
    • Alternative names
      • H3 K9 HMTase1 antibody
      • H3-K9-HMTase 1 antibody
      • Histone H3-K9 methyltransferase 1 antibody
      • Histone H3-K9 methyltransferase1 antibody
      • Histone lysine N methyltransferase, H3 lysine 9 specific 1 antibody
      • Histone-lysine N-methyltransferase SUV39H1 antibody
      • KMT1 A antibody
      • KMT1A antibody
      • Lysine N methyltransferase 1A antibody
      • Lysine N-methyltransferase 1A antibody
      • MG44 antibody
      • mIS6 antibody
      • Position-effect variegation 3-9 homolog antibody
      • Su(var)3 9 homolog 1 antibody
      • Su(var)3-9 homolog 1 antibody
      • Suppressor of variegation 3 9 homolog 1 (Drosophila) antibody
      • Suppressor of variegation 3-9 homolog 1 antibody
      • SUV39 H1 antibody
      • SUV39H antibody
      • SUV39H1 antibody
      • SUV91_HUMAN antibody
      see all

    Anti-KMT1A / SUV39H1 antibody images

    • All lanes : Anti-KMT1A / SUV39H1 antibody (ab38637) at 1/100 dilution

      Lane 1 : SAOS-2 cell lysate
      Lane 2 : NIH/3T3 cell lysate

      Predicted band size : 48 kDa
      Observed band size : 50 kDa (why is the actual band size different from the predicted?)
      Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
    • ab38637 at 1/50 staining human HEK293 cells by ICC/IF. The cells were formaldehyde fixed, blocked with BSA and incubated with the antibody for 1 hour. A goat anti-rabbit IgG was used as the secondary.

      See Abreview

    • ab38637 staining cultured human HeLa cells by ICC/IF.  Cells were PFA fixed and permeabilized in 0.5% Triton X100 prior to blocking in 5% BSA for 1 hour at 20°C.  The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 20°C.  A Cy3® conjugated donkey anti-rabbit antibody diluted 1/400 was used as the secondary.

      See Abreview

    References for Anti-KMT1A / SUV39H1 antibody (ab38637)

    This product has been referenced in:
    • Alkemade FE  et al. Prenatal exposure to apoE deficiency and postnatal hypercholesterolemia are associated with altered cell-specific lysine methyltransferase and histone methylation patterns in the vasculature. Am J Pathol 176:542-8 (2010). IHC-P ; Mouse . Read more (PubMed: 20035052) »

    See 1 Publication for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cultured Cells (HeLa cells)
    Specification HeLa cells
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.5% Triton X100
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

    Dr. Alexander Rapp

    Verified customer

    Submitted Apr 18 2008

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HEK293)
    Specification HEK293
    Fixative Formaldehyde
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10

    Abcam user community

    Verified customer

    Submitted Jan 15 2007