Anti-KMT1A / SUV39H1 antibody (ab97680)


  • Product nameAnti-KMT1A / SUV39H1 antibody
    See all KMT1A / SUV39H1 primary antibodies
  • Description
    Rabbit polyclonal to KMT1A / SUV39H1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region of Human KMT1A/ SUV39H1 (NP_003164)

  • Positive control
    • 721_B cell lysate



Our Abpromise guarantee covers the use of ab97680 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 48 kDa. Optimal concentration of antibody depends the protein level in a given sample. Our recommended concentrations are the starting point for general use. Customers are advised have to do a series of titration tests to determine optimal concentration. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.


  • FunctionHistone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys-9' as substrate. Also weakly methylates histone H1 (in vitro). H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin at pericentric and telomere regions. H3 'Lys-9' trimethylation is also required to direct DNA methylation at pericentric repeats. SUV39H1 is targeted to histone H3 via its interaction with RB1 and is involved in many processes, such as repression of MYOD1-stimulated differentiation, regulation of the control switch for exiting the cell cycle and entering differentiation, repression by the PML-RARA fusion protein, BMP-induced repression, repression of switch recombination to IgA and regulation of telomere length. Component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD(+)/NADP(+) ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. Recruited by the large PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1, contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation.
  • Sequence similaritiesBelongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar3-9 subfamily.
    Contains 1 chromo domain.
    Contains 1 post-SET domain.
    Contains 1 pre-SET domain.
    Contains 1 SET domain.
  • Developmental stageAccumulates during mitosis at centromeres during prometaphase, but dissociates from the centromere at the meta- to anaphase transition.
  • DomainAlthough the SET domain contains the active site of enzymatic activity, both pre-SET and post-SET domains are required for methyltransferase activity. The SET domain also participates to stable binding to heterochromatin.
    In the pre-SET domain, Cys residues bind 3 zinc ions that are arranged in a triangular cluster; some of these Cys residues contribute to the binding of two zinc ions within the cluster.
  • Post-translational
    Phosphorylated on serine residues, and to a lesser degree, on threonine residues. The phosphorylated form is stabilized by SBF1 and is less active in its transcriptional repressor function.
    Acetylated at Lys-266, leading to inhibition of enzyme activity. SIRT1-mediated deacetylation relieves this inhibition.
  • Cellular localizationNucleus. Nucleus lamina. Nucleus, nucleoplasm. Chromosome, centromere. Associates with centromeric constitutive heterochromatin.
  • Information by UniProt
  • Database links
  • Alternative names
    • H3 K9 HMTase1 antibody
    • H3-K9-HMTase 1 antibody
    • Histone H3-K9 methyltransferase 1 antibody
    • Histone H3-K9 methyltransferase1 antibody
    • Histone lysine N methyltransferase, H3 lysine 9 specific 1 antibody
    • Histone-lysine N-methyltransferase SUV39H1 antibody
    • KMT1 A antibody
    • KMT1A antibody
    • Lysine N methyltransferase 1A antibody
    • Lysine N-methyltransferase 1A antibody
    • MG44 antibody
    • mIS6 antibody
    • Position-effect variegation 3-9 homolog antibody
    • Su(var)3 9 homolog 1 antibody
    • Su(var)3-9 homolog 1 antibody
    • Suppressor of variegation 3 9 homolog 1 (Drosophila) antibody
    • Suppressor of variegation 3-9 homolog 1 antibody
    • SUV39 H1 antibody
    • SUV39H antibody
    • SUV39H1 antibody
    • SUV91_HUMAN antibody
    see all

Anti-KMT1A / SUV39H1 antibody images

  • Anti-KMT1A / SUV39H1 antibody (ab97680) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 48 kDa

References for Anti-KMT1A / SUV39H1 antibody (ab97680)

ab97680 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X100

Dr. Kirk McManus

Verified customer

Submitted Mar 15 2011