• Product nameAnti-KMT1B / SUV39H2 antibody
    See all KMT1B / SUV39H2 primary antibodies
  • Description
    Goat polyclonal to KMT1B / SUV39H2
  • Tested applicationsSuitable for: ChIP, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide:


    , corresponding to C terminal amino acids 398-410 of Human SUV39H2.

  • Positive control
    • Rat Kidney Lysate


Associated products


Our Abpromise guarantee covers the use of ab5264 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration. PubMed: 21060834
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 47 kDa.Can be blocked with Human KMT1B / SUV39H2 peptide (ab23082).


  • FunctionHistone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys-9' as substrate. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin at pericentric and telomere regions. H3 'Lys-9' trimethylation is also required to direct DNA methylation at pericentric repeats. SUV39H1 is targeted to histone H3 via its interaction with RB1 and is involved in many processes, such as cell cycle regulation, transcriptional repression and regulation of telomere length. May participate in regulation of higher order chromatin organization during spermatogenesis.
  • Sequence similaritiesBelongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily.
    Contains 1 chromo domain.
    Contains 1 post-SET domain.
    Contains 1 pre-SET domain.
    Contains 1 SET domain.
  • DomainAlthough the SET domain contains the active site of enzymatic activity, both pre-SET and post-SET domains are required for methyltransferase activity. The SET domain also participates to stable binding to heterochromatin.
  • Cellular localizationNucleus. Chromosome > centromere. Associates with centromeric constitutive heterochromatin.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ23414 antibody
    • H3 K9 HMTase 2 antibody
    • H3-K9-HMTase 2 antibody
    • Histone H3 K9 methyltransferase 2 antibody
    • Histone H3-K9 methyltransferase 2 antibody
    • Histone lysine N methyltransferase H3 lysine 9 specific 2 antibody
    • Histone lysine N methyltransferase SUV39H2 antibody
    • Histone-lysine N-methyltransferase SUV39H2 antibody
    • KMT1B antibody
    • Lysine N methyltransferase 1B antibody
    • Lysine N-methyltransferase 1B antibody
    • sSuppressor of variegation 3 9 homolog 2 (Drosophila) antibody
    • Su(var)3 9 Drosophila homolog of 2 antibody
    • Su(var)3 9 homolog 2 antibody
    • Su(var)3-9 homolog 2 antibody
    • Suppressor of variegation 3 9 homolog 2 antibody
    • Suppressor of variegation 3-9 homolog 2 antibody
    • Suv39h2 antibody
    • SUV92_HUMAN antibody
    see all

Anti-KMT1B / SUV39H2 antibody images

  • Anti-KMT1B / SUV39H2 antibody (ab5264) at 1 µg/ml + Rat Kidney Lysate in RIPA buffer at 35 µg
    developed using the ECL technique

    Predicted band size : 47 kDa

    Primary incubation 1 hour

References for Anti-KMT1B / SUV39H2 antibody (ab5264)

This product has been referenced in:
  • Salton M  et al. Identification by high-throughput imaging of the histone methyltransferase EHMT2 as an epigenetic regulator of VEGFA alternative splicing. Nucleic Acids Res 42:13662-73 (2014). ChIP ; Human . Read more (PubMed: 25414343) »
  • Li Q  et al. Polycomb CBX7 Directly Controls Trimethylation of Histone H3 at Lysine 9 at the p16 Locus. PLoS One 5:e13732 (2010). ChIP ; Human . Read more (PubMed: 21060834) »

See all 2 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - nuclear (MEF)
Loading amount 100 µg
Specification MEF
Gel Running Conditions Non-reduced Denaturing (gel 10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Mar 12 2009

Application ChIP on chip
Sample Mouse Cell lysate - nuclear (MEF)
Specification MEF
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldhehyde
Detection step Microarray

Abcam user community

Verified customer

Submitted May 30 2008

Thank you for your enquiry. To our knowledge, ab3659 has yet to be tested in IF. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will...

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