Overview

  • Product name
    Anti-KMT1B / SUV39H2 antibody
    See all KMT1B / SUV39H2 primary antibodies
  • Description
    Goat polyclonal to KMT1B / SUV39H2
  • Tested applications
    Suitable for: ChIP, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to Human KMT1B/ SUV39H2 aa 398-410 (C terminal).
    Sequence:

    CKCGAVTCRGYLN


    Database link: Q9H5I1
    (Peptide available as ab23082)

  • Positive control
    • Rat Kidney Lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab5264 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration. PubMed: 21060834
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 47 kDa.Can be blocked with Human KMT1B / SUV39H2 peptide (ab23082).

Target

  • Function
    Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys-9' as substrate. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin at pericentric and telomere regions. H3 'Lys-9' trimethylation is also required to direct DNA methylation at pericentric repeats. SUV39H1 is targeted to histone H3 via its interaction with RB1 and is involved in many processes, such as cell cycle regulation, transcriptional repression and regulation of telomere length. May participate in regulation of higher order chromatin organization during spermatogenesis.
  • Sequence similarities
    Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily.
    Contains 1 chromo domain.
    Contains 1 post-SET domain.
    Contains 1 pre-SET domain.
    Contains 1 SET domain.
  • Domain
    Although the SET domain contains the active site of enzymatic activity, both pre-SET and post-SET domains are required for methyltransferase activity. The SET domain also participates to stable binding to heterochromatin.
  • Cellular localization
    Nucleus. Chromosome > centromere. Associates with centromeric constitutive heterochromatin.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ23414 antibody
    • H3 K9 HMTase 2 antibody
    • H3-K9-HMTase 2 antibody
    • Histone H3 K9 methyltransferase 2 antibody
    • Histone H3-K9 methyltransferase 2 antibody
    • Histone lysine N methyltransferase H3 lysine 9 specific 2 antibody
    • Histone lysine N methyltransferase SUV39H2 antibody
    • Histone-lysine N-methyltransferase SUV39H2 antibody
    • KMT1B antibody
    • Lysine N methyltransferase 1B antibody
    • Lysine N-methyltransferase 1B antibody
    • sSuppressor of variegation 3 9 homolog 2 (Drosophila) antibody
    • Su(var)3 9 Drosophila homolog of 2 antibody
    • Su(var)3 9 homolog 2 antibody
    • Su(var)3-9 homolog 2 antibody
    • Suppressor of variegation 3 9 homolog 2 antibody
    • Suppressor of variegation 3-9 homolog 2 antibody
    • Suv39h2 antibody
    • SUV92_HUMAN antibody
    see all

Anti-KMT1B / SUV39H2 antibody images

  • Anti-KMT1B / SUV39H2 antibody (ab5264) at 1 µg/ml + Rat Kidney Lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 47 kDa

    Primary incubation 1 hour

References for Anti-KMT1B / SUV39H2 antibody (ab5264)

This product has been referenced in:
  • Yang G  et al. The histone H3K9 methyltransferase SUV39H links SIRT1 repression to myocardial infarction. Nat Commun 8:14941 (2017). ChIP . Read more (PubMed: 28361889) »
  • Salton M  et al. Identification by high-throughput imaging of the histone methyltransferase EHMT2 as an epigenetic regulator of VEGFA alternative splicing. Nucleic Acids Res 42:13662-73 (2014). ChIP ; Human . Read more (PubMed: 25414343) »

See all 3 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - nuclear (MEF)
Loading amount
100 µg
Specification
MEF
Gel Running Conditions
Non-reduced Denaturing (gel 10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Mar 12 2009

Application
ChIP on chip
Sample
Mouse Cell lysate - nuclear (MEF)
Specification
MEF
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldhehyde
Detection step
Microarray
Username

Abcam user community

Verified customer

Submitted May 30 2008

Thank you for your enquiry. To our knowledge, ab3659 has yet to be tested in IF. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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