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Anti-KMT1C / G9a antibody (ab31874)
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Proteins and Peptides
ab31873
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Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab31874? Please let us know so that we can cite the reference in this datasheet
ab31874 has been referenced in 2 publications.
- Sdek P et al. Rb and p130 control cell cycle gene silencing to maintain the postmitotic phenotype in cardiac myocytes. J Cell Biol 194:407-23 (2011). WB; Mouse. PubMed: 21825075
- Davison JM et al. Gestational choline supply regulates methylation of histone H3, expression of histone methyltransferases G9a (Kmt1c) and Suv39h1 (Kmt1a), and DNA methylation of their genes in rat fetal liver and brain. J Biol Chem 284:1982-9 (2009). WB; Rat. PubMed: 19001366
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - G9a antibody (ab31874)
Anti-KMT1C / G9a antibody (ab31874) at 1 µg/ml + Mouse EGL Whole Cell Lysate at 10 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size : 140 kDa
Observed band size : 140 kDa
Additional bands at : 170 kDa (possible isoform).
It is believed that the additional bands in the 170 kDa region are two splice variants (PMID: 12130538).
Tachibana M et al. G9a histone methyltransferase plays a dominant role in euchromatic histone H3 lysine 9 methylation and is essential for early embryogenesis. Genes Dev 16:1779-91 (2002).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT1C / G9a antibody (ab31874)
IHC image of ab31874 staining in mouse testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab31874, 5µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
KMT1C / G9a antibody for WB in Mouse (31874)
KMT1C / G9a antibody for WB in Human (31874)
KMT1C / G9a antibody for Immunoprecipitation in Human (31874)
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