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Overview
Product name
Anti-KMT1C / G9a antibody - ChIP Grade
See all KMT1C / G9a products (3) ...
Description
Rabbit polyclonal to KMT1C / G9a - ChIP Grade
Tested applications
IP, ChIP, IHC-P, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fusion protein, corresponding to amino acids 831-1001 of Human G9a
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Intracellular
Developmental Biology >> Reproduction >> Germ cell markers
Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Methylation >> Lysine methylation
Stem Cells >> Germline Stem Cells >> Embryonic Germ Cells
Stem Cells >> Embryonic Stem Cells >> Intracellular
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Methylation
Applications
Show applications key
Our Abpromise guarantee covers the use of ab40542 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at an assay dependent dilution.
ChIP: Use at an assay dependent dilution.
IHC-P: 1/2000Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: 1/2000 - 1/2500.Predicted molecular weight: 132 kDa.(For endogeneous detection (without G9a over expression): use nuclear extracts and block overnight or perform IP prior to WB.)
Target
Relevance
KMT1C / G9a is a histone methytransferase that preferentially methylates K9 and K27 of histone H3 in vitro. H3 K9 methylation serves as a mark for epigenetic transcriptional repression by recruiting HP1 to the site of this post-translational modification. The binding of HP1 also may serve to recruit Dnmt3a/b, resulting in DNA methylation. G9a-mediated histone methylation plays a significant role in epigenetic silencing.
Cellular localization
Nuclear
Alternative names
- Bat 8 antibody
- Bat8 antibody
- EC 2.1.1.43 antibody
see all
Database links
- Entrez Gene: 10919 Human
- Omim: 604599 Human
- SwissProt: Q96KQ7 Human
- Unigene: 75196 Human
Anti-KMT1C / G9a antibody - ChIP Grade images:
Western blot - G9a antibody - ChIP Grade (ab40542)
All lanes : Anti-KMT1C / G9a antibody - ChIP Grade (ab40542) at 1/2500 dilution
Lane 1 : Untransfected HeLa cell extract
Lane 2 : G9a transfected HeLa cell extract
Predicted band size : 132 kDa
Observed band size : 132 kDa
Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-KMT1C / G9a antibody - ChIP Grade(ab40542)
ab40542 (1:2000) staining KMT1C in human prostate using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
References for Anti-KMT1C / G9a antibody - ChIP Grade (ab40542)
This product has been referenced in:
- Stewart MDet al. Dynamic Regulation of Histone Modifications in Xenopus Oocytes through Histone Exchange. Mol Cell Biol 26:6890-901 (2006).Read more (PubMed: 16943430) »
- Purcell DJet al. A Distinct Mechanism for Coactivator versus Corepressor Function by Histone Methyltransferase G9a in Transcriptional Regulation. J Biol Chem 286:41963-71 (2011). ChIP; Human.Read more (PubMed: 21984853) »
See all 5 publications for this product
Publishing research using ab40542? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"