Anti-KMT1E / SETDB1 antibody (ab228575)
Key features and details
- Rabbit polyclonal to KMT1E / SETDB1
- Suitable for: WB, IP, ChIP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-KMT1E / SETDB1 antibody
See all KMT1E / SETDB1 primary antibodies -
Description
Rabbit polyclonal to KMT1E / SETDB1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ChIPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Recombinant fragment within Human KMT1E/ SETDB1 (intracellular). The exact sequence is proprietary.
Database link: Q15047 -
Positive control
- ChIP: HeLa chromatin extract. IP: HeLa nuclear extract. WB: HeLa whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab228575 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 143 kDa.
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IP |
1/100 - 1/500.
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ChIP |
Use at an assay dependent concentration.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 143 kDa. |
IP
1/100 - 1/500. |
ChIP
Use at an assay dependent concentration. |
Target
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Function
Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 'Lys-9' trimethylation is coordinated with DNA methylation. Probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins. -
Tissue specificity
Widely expressed. High expression in testis. -
Sequence similarities
Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily.
Contains 1 MBD (methyl-CpG-binding) domain.
Contains 1 post-SET domain.
Contains 1 pre-SET domain.
Contains 1 SET domain.
Contains 2 Tudor domains. -
Domain
The pre-SET, SET and post-SET domains are all required for methyltransferase activity. The 347-amino-acid insertion in the SET domain has no effect on the catalytic activity.
Isoform 2 lacks all domains required for histone methyltransferase activity. -
Cellular localization
Nucleus. Chromosome. Associated with non-pericentromeric regions of chromatin. Excluded from nucleoli and islands of condensed chromatin. - Information by UniProt
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Database links
- Entrez Gene: 100138100 Cow
- Entrez Gene: 9869 Human
- Entrez Gene: 84505 Mouse
- Entrez Gene: 689883 Rat
- Omim: 604396 Human
- SwissProt: Q15047 Human
- SwissProt: O88974 Mouse
- SwissProt: D4A081 Rat
see all -
Alternative names
- AU022152 antibody
- EC 2.1.1.43 antibody
- ERG-associated protein with SET domain antibody
see all
Images
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Anti-KMT1E / SETDB1 antibody (ab228575) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 143 kDa5% SDS-PAGE
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KMT1E / SETDB1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) nuclear extract using 4 µg of ab228575. Western blot was performed from the immunoprecipitate using ab228575 at 1/1000 dilution.
Lane 1: Control IgG IP in HeLa nuclear extract.
Lane 2: ab228575 IP in HeLa nuclear extract.
5% SDS-PAGE
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Cross-linked ChIP was performed with HeLa (human epithelial cell line from cervix adenocarcinoma) chromatin extract and 5 μg of either control rabbit IgG or ab228575. The precipitated DNA was detected by PCR with primer set targeting to p53P2.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab228575 has not yet been referenced specifically in any publications.