Overview

  • Product nameAnti-KMT1E / SETDB1 antibody
    See all KMT1E / SETDB1 primary antibodies
  • Description
    Rabbit polyclonal to KMT1E / SETDB1
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Dog, Pig
  • Immunogen

    Synthetic peptide:

    SRNYGYNPSPVKPEGLRR

    conjugated to KLH, corresponding to amino acids 1058-1075 of Human KMT1E/ SETDB1.

  • Positive control
    • Human testis tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab115340 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2500. Predicted molecular weight: 143 kDa.
ELISA 1/10000 - 1/50000.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionHistone methyltransferase that specifically trimethylates 'Lys-9' of histone H3. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 'Lys-9' trimethylation is coordinated with DNA methylation. Probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins.
  • Tissue specificityWidely expressed. High expression in testis.
  • Sequence similaritiesBelongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily.
    Contains 1 MBD (methyl-CpG-binding) domain.
    Contains 1 post-SET domain.
    Contains 1 pre-SET domain.
    Contains 1 SET domain.
    Contains 2 Tudor domains.
  • DomainThe pre-SET, SET and post-SET domains are all required for methyltransferase activity. The 347-amino-acid insertion in the SET domain has no effect on the catalytic activity.
    Isoform 2 lacks all domains required for histone methyltransferase activity.
  • Cellular localizationNucleus. Chromosome. Associated with non-pericentromeric regions of chromatin. Excluded from nucleoli and islands of condensed chromatin.
  • Information by UniProt
  • Database links
  • Alternative names
    • AU022152 antibody
    • EC 2.1.1.43 antibody
    • ERG-associated protein with SET domain antibody
    • ESET antibody
    • H3 K9 HMTase 4 antibody
    • H3-K9-HMTase 4 antibody
    • H3-K9-HMTase4 antibody
    • Histone H3 K9 methyltransferase 4 antibody
    • Histone H3-K9 methyltransferase 4 antibody
    • Histone-lysine N-methyltransferase SETDB1 antibody
    • KG1T antibody
    • KIAA0067 antibody
    • KMT1E antibody
    • Lysine N-methyltransferase 1E antibody
    • MGC90670 antibody
    • mKIAA0067 antibody
    • SET domain bifurcated 1 antibody
    • Set domain protein, bifurcated, 1 antibody
    • SETB1_HUMAN antibody
    • Setdb1 antibody
    see all

Anti-KMT1E / SETDB1 antibody images

  • ab115340 at 5ug/ml staining KMT1E/SETDB1 in Formalin-fixed, Paraffin-embedded Human testis tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

References for Anti-KMT1E / SETDB1 antibody (ab115340)

ab115340 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab115340.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"