Overview

  • Product name
    Anti-KMT1E / SETDB1 antibody
    See all KMT1E / SETDB1 primary antibodies
  • Description
    Rabbit polyclonal to KMT1E / SETDB1
  • Specificity
    Detects a band at 170kD in 293 cell lysate. This band can be blocked by the immunising peptide in WB. The predicted size is 143kD, however, several other antibodies detect bands at the same size as ab5430.
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    C-SRNYGYNPSPVKPEG

    conjugated to KLH, corresponding to amino acids 1058-1072 of Human ESET.

Properties

Applications

Our Abpromise guarantee covers the use of ab5430 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB 1/1000. Detects a band of approximately 170 kDa (predicted molecular weight: 143 kDa).

Target

  • Function
    Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 'Lys-9' trimethylation is coordinated with DNA methylation. Probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins.
  • Tissue specificity
    Widely expressed. High expression in testis.
  • Sequence similarities
    Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily.
    Contains 1 MBD (methyl-CpG-binding) domain.
    Contains 1 post-SET domain.
    Contains 1 pre-SET domain.
    Contains 1 SET domain.
    Contains 2 Tudor domains.
  • Domain
    The pre-SET, SET and post-SET domains are all required for methyltransferase activity. The 347-amino-acid insertion in the SET domain has no effect on the catalytic activity.
    Isoform 2 lacks all domains required for histone methyltransferase activity.
  • Cellular localization
    Nucleus. Chromosome. Associated with non-pericentromeric regions of chromatin. Excluded from nucleoli and islands of condensed chromatin.
  • Information by UniProt
  • Database links
  • Alternative names
    • AU022152 antibody
    • EC 2.1.1.43 antibody
    • ERG-associated protein with SET domain antibody
    • ESET antibody
    • H3 K9 HMTase 4 antibody
    • H3-K9-HMTase 4 antibody
    • H3-K9-HMTase4 antibody
    • Histone H3 K9 methyltransferase 4 antibody
    • Histone H3-K9 methyltransferase 4 antibody
    • Histone-lysine N-methyltransferase SETDB1 antibody
    • KG1T antibody
    • KIAA0067 antibody
    • KMT1E antibody
    • Lysine N-methyltransferase 1E antibody
    • MGC90670 antibody
    • mKIAA0067 antibody
    • SET domain bifurcated 1 antibody
    • Set domain protein, bifurcated, 1 antibody
    • SETB1_HUMAN antibody
    • Setdb1 antibody
    see all

Images



  • Predicted band size : 143 kDa


    Western blot using ab5430 at 1/1000 on 293 cell lysate. 30µg of lysate per lane. Peptide competition (not shown) blocks staining of this band. Predicted size is 143kD, but other antibodies also show staining at about 170kD.
  • ICC/IF image of ab5430 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5430, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Freiburghaus C  et al. Identification of ubiquitin in bovine milk and its growth inhibitory effects on human cancer cell lines. J Dairy Sci 93:3442-52 (2010). Read more (PubMed: 20655412) »
  • Freiburghaus C  et al. Lactoferricin treatment decreases the rate of cell proliferation of a human colon cancer cell line. J Dairy Sci 92:2477-84 (2009). Read more (PubMed: 19447979) »

See all 2 Publications for this product

Customer reviews and Q&As

Application
Immunoprecipitation
Sample
Human Cell lysate - nuclear (HeLa, K562 and 293T(human embryonic kidney))
Specification
HeLa, K562 and 293T(human embryonic kidney)
Immuno-precipitation step
Protein A
Username

Abcam user community

Verified customer

Submitted Oct 28 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application
ChIP
Sample
Mouse Cell lysate - nuclear (mouse ES cells)
Specification
mouse ES cells
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 15 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde
Detection step
Real-time PCR
Positive control
Competitor Antibody
Username

Dr. Steve Bilodeau

Verified customer

Submitted Oct 16 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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