Anti-KMT2B / MLL4 antibody (ab104444)
Key features and details
- Goat polyclonal to KMT2B / MLL4
- Suitable for: ELISA, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-KMT2B / MLL4 antibody
See all KMT2B / MLL4 primary antibodies -
Description
Goat polyclonal to KMT2B / MLL4 -
Host species
Goat -
Tested applications
Suitable for: ELISA, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig, Monkey, Zebrafish, Gorilla, Common marmoset -
Immunogen
Synthetic peptide corresponding to Human KMT2B/ MLL4 aa 2703-2715 (C terminal). Entrez gene ID: 9757
Sequence:CNCGAKRCRRFLN
Database link: Q9UMN6 -
Positive control
- IHC-P: Human brain cortex and testis tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Immunoaffinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab104444 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ELISA
Use at an assay dependent concentration. |
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Histone methyltransferase. Methylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in beta-globin locus transcription regulation by being recruited by NFE2. Plays an important role in controlling bulk H3K4me during oocyte growth and preimplantation development. Required during the transcriptionally active period of oocyte growth for the establishment and/or maintenance of bulk H3K4 trimethylation (H3K4me3), global transcriptional silencing that preceeds resumption of meiosis, oocyte survival and normal zygotic genome activation. -
Tissue specificity
Widely expressed. Highest levels in testis. Also found in brain, bone marrow, heart, muscle, kidney, placenta, spleen, thymus, prostate, ovary, intestine, colon, peripheral blood lymphocytes and pancreas. Often amplified in pancreatic carcinomas. -
Sequence similarities
Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. TRX/MLL subfamily.
Contains 3 A.T hook DNA-binding domains.
Contains 1 CXXC-type zinc finger.
Contains 1 FYR C-terminal domain.
Contains 1 FYR N-terminal domain.
Contains 3 PHD-type zinc fingers.
Contains 1 post-SET domain.
Contains 1 SET domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 9757 Human
- Entrez Gene: 75410 Mouse
- Entrez Gene: 102550344 Rat
- Omim: 606834 Human
- SwissProt: Q9UMN6 Human
- SwissProt: O08550 Mouse
- Unigene: 676457 Human
- Unigene: 92236 Human
see all -
Alternative names
- Histone-lysine N-methyltransferase 2B antibody
- HRX2 antibody
- KIAA0304 gene product antibody
see all
Images
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ab104444, at 5 µg/ml, staining KMT2B / MLL4 in formalin-fixed, paraffin-embedded human brain cortex tissue by Immunohistochemistry. A biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen was used as the secondary antibody.
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ab104444, at 5 µg/ml, staining KMT2B / MLL4 in formalin-fixed, paraffin-embedded human testis tissue by Immunohistochemistry. A biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen was used as the secondary antibody.
Protocols
Datasheets and documents
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Datasheet download
References (3)
ab104444 has been referenced in 3 publications.
- Swaminathan J et al. Cross-Talk Between Histone Methyltransferases and Demethylases Regulate REST Transcription During Neurogenesis. Front Oncol 12:855167 (2022). PubMed: 35600406
- Su CH et al. Regulation of IL-20 Expression by Estradiol through KMT2B-Mediated Epigenetic Modification. PLoS One 11:e0166090 (2016). ChIP, IHC-P ; Human . PubMed: 27806114
- Fulcoli FG et al. Rebalancing gene haploinsufficiency in vivo by targeting chromatin. Nat Commun 7:11688 (2016). PubMed: 27256596