Anti-KMT2B / MLL4 antibody (ab104444)

Overview

  • Product nameAnti-KMT2B / MLL4 antibody
    See all KMT2B / MLL4 primary antibodies
  • Description
    Goat polyclonal to KMT2B / MLL4
  • Tested applicationsSuitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig, Monkey, Zebrafish, Gorilla, Marmoset (common)
  • Immunogen

    Synthetic peptide corresponding to Human KMT2B/ MLL4 aa 2703-2715 (C terminal). Entrez gene ID: 9757
    Sequence:

    CNCGAKRCRRFLN


    Database link: Q9UMN6

  • Positive control
    • Human Brain, Cortex; Human Testis

Properties

Applications

Our Abpromise guarantee covers the use of ab104444 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/128000.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration.

Target

  • FunctionHistone methyltransferase. Methylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in beta-globin locus transcription regulation by being recruited by NFE2. Plays an important role in controlling bulk H3K4me during oocyte growth and preimplantation development. Required during the transcriptionally active period of oocyte growth for the establishment and/or maintenance of bulk H3K4 trimethylation (H3K4me3), global transcriptional silencing that preceeds resumption of meiosis, oocyte survival and normal zygotic genome activation.
  • Tissue specificityWidely expressed. Highest levels in testis. Also found in brain, bone marrow, heart, muscle, kidney, placenta, spleen, thymus, prostate, ovary, intestine, colon, peripheral blood lymphocytes and pancreas. Often amplified in pancreatic carcinomas.
  • Sequence similaritiesBelongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. TRX/MLL subfamily.
    Contains 3 A.T hook DNA-binding domains.
    Contains 1 CXXC-type zinc finger.
    Contains 1 FYR C-terminal domain.
    Contains 1 FYR N-terminal domain.
    Contains 3 PHD-type zinc fingers.
    Contains 1 post-SET domain.
    Contains 1 SET domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Histone-lysine N-methyltransferase 2B antibody
    • HRX2 antibody
    • KIAA0304 gene product antibody
    • KMT2B antibody
    • KMT2B_HUMAN antibody
    • Likely ortholog of mouse WW domain binding protein 7 antibody
    • Lysine N-methyltransferase 2B antibody
    • mixed lineage leukemia 4 antibody
    • Mixed lineage leukemia gene homolog 2 protein antibody
    • MLL2 antibody
    • myeloid lymphoid leukemia 4 antibody
    • Myeloid lymphoid or mixed lineage leukemia protein 4 antibody
    • Myeloid/lymphoid or mixed-lineage leukemia protein 4 antibody
    • Trithorax homolog 2 antibody
    • trithorax homologue 2 antibody
    • TRX2 antibody
    • WBP-7 antibody
    • WBP7 antibody
    • WW domain-binding protein 7 antibody
    see all

Anti-KMT2B / MLL4 antibody images

  • ab104444, at 5 µg/ml, staining KMT2B / MLL4 in formalin-fixed, paraffin-embedded Human Brain, Cortex by Immunohistochemistry, using a biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

  • ab104444, at 5 µg/ml, staining KMT2B / MLL4 in formalin-fixed, paraffin-embedded Human Testis by Immunohistochemistry using a biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

References for Anti-KMT2B / MLL4 antibody (ab104444)

ab104444 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (5%)
Sample Mouse Cell lysate - whole cell (primary bone marrow derived macrophages)
Specification primary bone marrow derived macrophages
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Sep 10 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"