Our Abpromise guarantee covers the use of ab15962 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 4 - 8 µg/ml.


  • FunctionHistone methyltransferase. Methylates 'Lys-4' of histone H3 (H3K4me). H3K4me represents a specific tag for epigenetic transcriptional activation. Plays a central role in beta-globin locus transcription regulation by being recruited by NFE2. Acts as a coactivator for estrogen receptor by being recruited by ESR1, thereby activating transcription. Plays an important role in controlling bulk H3K4me during oocyte growth and preimplantation development. Required during the transcriptionally active period of oocyte growth for the establishment and/or maintenance of bulk H3K4 trimethylation (H3K4me3), global transcriptional silencing that preceeds resumption of meiosis, oocyte survival and normal zygotic genome activation.
  • Tissue specificityExpressed in most adult tissues, including a variety of hematoipoietic cells, with the exception of the liver.
  • Involvement in diseaseDefects in MLL2 are the cause of Kabuki syndrome (KABS) [MIM:147920]. It is a congenital mental retardation syndrome with additional features, including postnatal dwarfism, a peculiar facies characterized by long palpebral fissures with eversion of the lateral third of the lower eyelids, a broad and depressed nasal tip, large prominent earlobes, a cleft or high-arched palate, scoliosis, short fifth finger, persistence of fingerpads, radiographic abnormalities of the vertebrae, hands, and hip joints, and recurrent otitis media in infancy.
  • Sequence similaritiesBelongs to the histone-lysine methyltransferase family. TRX/MLL subfamily.
    Contains 1 FY-rich C-terminal domain.
    Contains 1 FY-rich N-terminal domain.
    Contains 5 PHD-type zinc fingers.
    Contains 1 post-SET domain.
    Contains 4 RING-type zinc fingers.
    Contains 1 SET domain.
  • DomainLXXLL motifs 5 and 6 are essential for the association with ESR1 nuclear receptor.
  • Post-translational
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AAD10 antibody
    • ALL1 related gene antibody
    • ALL1-related protein antibody
    • ALR antibody
    • CAGL114 antibody
    • Histone-lysine N-methyltransferase MLL2 antibody
    • KABUK1 antibody
    • Kabuki make up syndrome antibody
    • Kabuki mental retardation syndrome antibody
    • KMS antibody
    • KMT2B antibody
    • KMT2D antibody
    • Lysine N methyltransferase 2D antibody
    • Lysine N-methyltransferase 2B antibody
    • MLL2 antibody
    • MLL2_HUMAN antibody
    • MLL4 antibody
    • Myeloid/lymphoid or mixed lineage leukemia 2 antibody
    • Myeloid/lymphoid or mixed-lineage leukemia protein 2 antibody
    • TNRC21 antibody
    • Trinucleotide repeat containing 21 antibody
    see all

Anti-KMT2D / MLL2 antibody images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human placenta tissue, staining KMT2B / MLL2 with ab15962 at 4 µg/ml. Antigen retrieval was performed by heat mediation in a citrate buffer (pH 6).
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human liver tissue, staining KMT2B / MLL2 with ab15962 at 3.8 µg/ml. Antigen retrieval was performed by heat mediation in a citrate buffer (pH 6).

References for Anti-KMT2D / MLL2 antibody (ab15962)

This product has been referenced in:
  • Pradeepa MM  et al. Psip1/Ledgf p75 restrains Hox gene expression by recruiting both trithorax and polycomb group proteins. Nucleic Acids Res 42:9021-32 (2014). ChIP . Read more (PubMed: 25056311) »

See 1 Publication for this product

Product Wall

Application ChIP
Sample Human Cell lysate - nuclear (K562 (human erythroid cell line))
Negative control Normal IgG
Specification K562 (human erythroid cell line)
Detection step Taqman
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Positive control GATA-1 antibody

Abcam user community

Verified customer

Submitted Oct 20 2015

The antibody incubation time and temperature was 1h at ambient temp for IHC-P - for both images.

Thank you for your email. As far as I know, there are currently no plans for production of a MLL4 antibody. I suggest checking back with us in the future, and if you have any additional questions, please do let us know.