Anti-KMT2G / hSET1B antibody (ab112049)

Overview

  • Product name
    Anti-KMT2G / hSET1B antibody
    See all KMT2G / hSET1B primary antibodies
  • Description
    Rabbit polyclonal to KMT2G / hSET1B
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Cow, Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide, corresponding to a region between amino acids 350-400 of KMT2G/ hSET1B (NP_055863.1).

  • Positive control
    • Human Breast Carcinoma, Human Ovarian Carcinoma, and Human Testicular Seminoma tissues

Properties

Applications

Our Abpromise guarantee covers the use of ab112049 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.

Target

  • Function
    Histone methyltransferase that specifically methylates 'Lys-4' of histone H3, when part of the SET1 histone methyltransferase (HMT) complex, but not if the neighboring 'Lys-9' residue is already methylated. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. The non-overalpping localization with SETD1A suggests that SETD1A and SETD1B make non-redundant contributions to the epigenetic control of chromatin structure and gene expression. Specifically tri-methylates 'Lys-4' of histone H3 in vitro.
  • Sequence similarities
    Contains 1 post-SET domain.
    Contains 1 RRM (RNA recognition motif) domain.
    Contains 1 SET domain.
  • Cellular localization
    Nucleus speckle. Chromosome. Localizes to a largely non-overlapping set of euchromatic nuclear speckles with SETD1A, suggesting that SETD1A and SET1B each bind to a unique set of target genes.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ20803 antibody
    • Histone-lysine N-methyltransferase SETD1B antibody
    • hSET1B antibody
    • KIAA1076 antibody
    • KMT2G antibody
    • Lysine N-methyltransferase 2G antibody
    • SET domain containing 1B antibody
    • SET domain-containing protein 1B antibody
    • SET1B antibody
    • SET1B_HUMAN antibody
    • SETD1B antibody
    see all

Images

  • Staining of Human KMT2G / hSET1B in a Formalin/PFA-fixed paraffin-embedded section of Human Breast Carcinoma using ab112049 at a dilution of 1/250.
  • Staining of Human KMT2G / hSET1B in a Formalin/PFA-fixed paraffin-embedded section of Human Ovarian Carcinoma using ab112049 at a dilution of 1/250.

References

ab112049 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% TritonX100 in PBS
Specification
HeLa
Fixative
Paraformaldehyde
Username

Dr. Kirk Mcmanus

Verified customer

Submitted Aug 07 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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