• Product name
    Anti-KMT2G / hSET1B antibody
    See all KMT2G / hSET1B primary antibodies
  • Description
    Rabbit polyclonal to KMT2G / hSET1B
  • Tested applications
    Suitable for: IHC-P, IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Chimpanzee, Rhesus monkey, Gorilla
  • Immunogen

    Synthetic peptide corresponding to Human KMT2G/ hSET1B aa 1400-1450.
    Database link: 23067

  • Positive control
    • HeLa whole cell lysate



Our Abpromise guarantee covers the use of ab113964 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      Histone methyltransferase that specifically methylates 'Lys-4' of histone H3, when part of the SET1 histone methyltransferase (HMT) complex, but not if the neighboring 'Lys-9' residue is already methylated. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. The non-overalpping localization with SETD1A suggests that SETD1A and SETD1B make non-redundant contributions to the epigenetic control of chromatin structure and gene expression. Specifically tri-methylates 'Lys-4' of histone H3 in vitro.
    • Sequence similarities
      Contains 1 post-SET domain.
      Contains 1 RRM (RNA recognition motif) domain.
      Contains 1 SET domain.
    • Cellular localization
      Nucleus speckle. Chromosome. Localizes to a largely non-overlapping set of euchromatic nuclear speckles with SETD1A, suggesting that SETD1A and SET1B each bind to a unique set of target genes.
    • Information by UniProt
    • Database links
    • Alternative names
      • FLJ20803 antibody
      • Histone-lysine N-methyltransferase SETD1B antibody
      • hSET1B antibody
      • KIAA1076 antibody
      • KMT2G antibody
      • Lysine N-methyltransferase 2G antibody
      • SET domain containing 1B antibody
      • SET domain-containing protein 1B antibody
      • SET1B antibody
      • SET1B_HUMAN antibody
      • SETD1B antibody
      see all

    Anti-KMT2G / hSET1B antibody images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling KMT2G / hSET1B with ab113964 at 1/1000 (1µg/ml). Detection: DAB.
    • Detection of KMT2G / hSET1B in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab113964 at 10 µg/mg lysate for IP (Lane 1). For WB detection an anti-KMT2G / hSET1B antibody (ab113984) which recognizes an upstream epitope was used at 1 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 3 minutes.

      Predicted band size : 209 kDa.

    References for Anti-KMT2G / hSET1B antibody (ab113964)

    ab113964 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab113964.
    Please use the links above to contact us or submit feedback about this product.


    Sign up