Overview

  • Product name
    Anti-KMT2G / hSET1B antibody
    See all KMT2G / hSET1B primary antibodies
  • Description
    Rabbit polyclonal to KMT2G / hSET1B
  • Tested applications
    Suitable for: IHC-P, IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Chimpanzee, Rhesus monkey, Gorilla
  • Immunogen

    Synthetic peptide corresponding to Human KMT2G/ hSET1B aa 1400-1450.
    Database link: 23067

  • Positive control
    • HeLa whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab113964 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      Histone methyltransferase that specifically methylates 'Lys-4' of histone H3, when part of the SET1 histone methyltransferase (HMT) complex, but not if the neighboring 'Lys-9' residue is already methylated. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. The non-overalpping localization with SETD1A suggests that SETD1A and SETD1B make non-redundant contributions to the epigenetic control of chromatin structure and gene expression. Specifically tri-methylates 'Lys-4' of histone H3 in vitro.
    • Sequence similarities
      Contains 1 post-SET domain.
      Contains 1 RRM (RNA recognition motif) domain.
      Contains 1 SET domain.
    • Cellular localization
      Nucleus speckle. Chromosome. Localizes to a largely non-overlapping set of euchromatic nuclear speckles with SETD1A, suggesting that SETD1A and SET1B each bind to a unique set of target genes.
    • Information by UniProt
    • Database links
    • Alternative names
      • FLJ20803 antibody
      • Histone-lysine N-methyltransferase SETD1B antibody
      • hSET1B antibody
      • KIAA1076 antibody
      • KMT2G antibody
      • Lysine N-methyltransferase 2G antibody
      • SET domain containing 1B antibody
      • SET domain-containing protein 1B antibody
      • SET1B antibody
      • SET1B_HUMAN antibody
      • SETD1B antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling KMT2G / hSET1B with ab113964 at 1/1000 (1µg/ml). Detection: DAB.
    • Detection of KMT2G / hSET1B in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab113964 at 10 µg/mg lysate for IP (Lane 1). For WB detection an anti-KMT2G / hSET1B antibody (ab113984) which recognizes an upstream epitope was used at 1 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 3 minutes.

      Predicted band size : 209 kDa.

    References

    ab113964 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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