The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use at an assay dependent concentration.
ab113984 has not performed satisfactorily when used for WB of KMT2G / hSET1B in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of KMT2G / hSET1B.
Use at 10 µg/mg of lysate.
Histone methyltransferase that specifically methylates 'Lys-4' of histone H3, when part of the SET1 histone methyltransferase (HMT) complex, but not if the neighboring 'Lys-9' residue is already methylated. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. The non-overalpping localization with SETD1A suggests that SETD1A and SETD1B make non-redundant contributions to the epigenetic control of chromatin structure and gene expression. Specifically tri-methylates 'Lys-4' of histone H3 in vitro.
ab113984 used at 10 µg/mg of lysate staining KMT2G / hSET1B in HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) by immunoprecipitation. For blotting immunoprecipitated KMT2G / hSET1B, ab113984 was used at 1 µg/ml.
Detection: Chemiluminescence with an exposure time of 3