Anti-KMT3A / HYPB / HIF-1 antibody (ab31358)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab31358 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250. Detects a band of approximately 288 kDa (predicted molecular weight: 288 kDa).

Target

  • FunctionHistone methyltransferase that methylates 'Lys-36' of histone H3. H3 'Lys-36' methylation represents a specific tag for epigenetic transcriptional activation. Probably plays a role in chromatin structure modulation during elongation via its interaction with hyperphosphorylated POLR2A. Binds DNA at promoters. May also act as a transcription activator that binds to promoters. Binds to the promoters of adenovirus 12 E1A gene in case of infection, possibly leading to regulate its expression.
  • Tissue specificityUbiquitously expressed.
  • Sequence similaritiesBelongs to the histone-lysine methyltransferase family. SET2 subfamily.
    Contains 1 AWS domain.
    Contains 1 post-SET domain.
    Contains 1 SET domain.
    Contains 1 WW domain.
  • DomainThe low charge region mediates the transcriptional activation activity.
  • Post-translational
    modifications
    May be automethylated.
  • Cellular localizationNucleus. Chromosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • EC 2.1.1.43 antibody
    • FLJ16420 antibody
    • FLJ22472 antibody
    • FLJ23184 antibody
    • FLJ45883 antibody
    • HBP231 antibody
    • HIF 1 antibody
    • HIF-1 antibody
    • HIF1 antibody
    • HIP-1 antibody
    • Histone lysine N methyltransferase SETD2 antibody
    • Histone-lysine N-methyltransferase SETD2 antibody
    • hSET2 antibody
    • HSPC069 antibody
    • Huntingtin interacting protein 1 antibody
    • Huntingtin interacting protein antibody
    • Huntingtin interacting protein B antibody
    • Huntingtin interacting protein HYPB antibody
    • Huntingtin yeast partner B antibody
    • Huntingtin-binding protein, 231-KD antibody
    • Huntingtin-interacting protein 1 antibody
    • Huntingtin-interacting protein B antibody
    • HYPB antibody
    • KIAA1732 antibody
    • KMT3A antibody
    • Lysine N methyltransferase 3A antibody
    • Lysine N-methyltransferase 3A antibody
    • p231HBP antibody
    • SET domain containing 2 antibody
    • SET domain-containing protein 2 antibody
    • SET2 antibody
    • SETD2 antibody
    • SETD2_HUMAN antibody
    see all

Anti-KMT3A / HYPB / HIF-1 antibody images

  • All lanes : Anti-KMT3A / HYPB / HIF-1 antibody (ab31358) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 288 kDa
    Observed band size : 288 kDa
    Additional bands at : 110 kDa,130 kDa,30 kDa,45 kDa,60 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes

    This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab31358 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

References for Anti-KMT3A / HYPB / HIF-1 antibody (ab31358)

This product has been referenced in:
  • Simon JM  et al. Variation in chromatin accessibility in human kidney cancer links H3K36 methyltransferase loss with widespread RNA processing defects. Genome Res 24:241-50 (2014). Human . Read more (PubMed: 24158655) »
  • Pfister SX  et al. SETD2-Dependent Histone H3K36 Trimethylation Is Required for Homologous Recombination Repair and Genome Stability. Cell Rep 7:2006-18 (2014). WB, ChIP ; Human . Read more (PubMed: 24931610) »

See all 4 Publications for this product

Product Wall

Thank you for your reply.

Yes, please keep me updated on the results with the controls. Should the results not improve, we can discuss options of free of charge replacement, credit or refund to resolve this case.

I wish you good l...

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Thank you for contacting us.

I am sorry to hear that the antibody is not giving you the correct band.

The human protein has3 isoforms(http://www.uniprot.org/uniprot/Q9BYW2), but none of these match the band size you are seeing. Read More

Thank you for your inquiry.

Most antibodies will work fine with either BSA or milk.

When testing our antibodies, our lab uses 5% BSA as a blocking reagent, so I recommend this.

Some antibodies give stronger, more specific ...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Placenta)
Specification Placenta
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Target Retrieval Solution
Permeabilization No
Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

Submitted Nov 17 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (fibroblast)
Loading amount 20 µg
Specification fibroblast
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Sep 26 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"