Synthetic peptide conjugated to KLH derived from within residues 500 - 600 of Human HYPB/ HIF-1.
(Peptide available as ab32693.)
Our Abpromise guarantee covers the use of ab31358 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/250. Detects a band of approximately 288 kDa (predicted molecular weight: 288 kDa).|
This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab31358 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.