FunctionProtein-lysine N-methyltransferase that methylates both histones and non-histone proteins. Specifically methylates histone H3 'Lys-4' (H3K4me) and dimethylates histone H3 'Lys-36' (H3K36me2). Has also methyltransferase activity toward non-histone proteins such as p53/TP53 and RB1. Monomethylates 'Lys-370' of p53/TP53, leading to decreased DNA-binding activity and subsequent transcriptional regulation activity of p53/TP53. Monomethylates 'Lys-860' of RB1/RB.
Sequence similaritiesContains 1 MYND-type zinc finger. Contains 1 SET domain.
All lanes : Anti-KMT3C / SMYD2 antibody (ab108217) at 0.1 µg/ml
Lane 1 : HeLa whole cell
lysate at 50 µg Lane 2 : HeLa whole cell
lysate at 15 µg Lane 3 : HeLa whole cell
lysate at 5 µg Lane 4 : 293T whole cell
lysate at 50 µg Lane 5 : NIH 3T3 whole cell
lysate at 50 µg
Developed using the ECL technique
Predicted band size : 50 kDa
Exposure time : 3 minutes
Western blot - Anti-KMT3C / SMYD2 antibody (ab108217)This image is courtesy of an anonymous Abreview
All lanes : Anti-KMT3C / SMYD2 antibody (ab108217) at 1/1000 dilution
Detection of KMT3C / SMYD2 by Western blot of Immunprecipitate.
ab108217, at 1 µg/ml, staining KMT3C / SMYD2 in HeLa whole cell lysate immunoprecipitated using ab108217 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with an exposure time of 10 seconds.
References for Anti-KMT3C / SMYD2 antibody (ab108217)
has not yet been referenced specifically in any publications.
Publishing research using ab108217? Please let us know so that we can cite the reference in this datasheet.
Concentration of lot no. is
Concentration not available for this lot.
Find concentration of your lot:
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"