Anti-KMT3C / SMYD2 antibody (ab108217)

Overview

  • Product nameAnti-KMT3C / SMYD2 antibody
    See all KMT3C / SMYD2 primary antibodies
  • Description
    Rabbit polyclonal to KMT3C / SMYD2
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Rhesus monkey, Gorilla, Orangutan, Platypus (Ornithorhynchus anatinus)
  • Immunogen

    Synthetic peptide corresponding to a region between residue 383 and 433 of Human KMT3C/ SMYD2 (NP_064582.2).

  • Positive control
    • HeLa, 293T and NIH 3T3 whole cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab108217 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 50 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • FunctionProtein-lysine N-methyltransferase that methylates both histones and non-histone proteins. Specifically methylates histone H3 'Lys-4' (H3K4me) and dimethylates histone H3 'Lys-36' (H3K36me2). Has also methyltransferase activity toward non-histone proteins such as p53/TP53 and RB1. Monomethylates 'Lys-370' of p53/TP53, leading to decreased DNA-binding activity and subsequent transcriptional regulation activity of p53/TP53. Monomethylates 'Lys-860' of RB1/RB.
  • Sequence similaritiesContains 1 MYND-type zinc finger.
    Contains 1 SET domain.
  • Cellular localizationCytoplasm > cytosol. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Histone methyltransferase SMYD2 antibody
    • HSKM B antibody
    • HSKM-B antibody
    • HSKMB antibody
    • KMT3C antibody
    • Lysine N-methyltransferase 3C antibody
    • MGC119305 antibody
    • N lysine methyltransferase SMYD2 antibody
    • N-lysine methyltransferase SMYD2 antibody
    • SET and MYND domain containing 2 antibody
    • SET and MYND domain containing protein 2 antibody
    • SET and MYND domain-containing protein 2 antibody
    • Smyd2 antibody
    • SMYD2_HUMAN antibody
    • Zinc finger MYND domain containing 14 antibody
    • ZMYND14 antibody
    see all

Anti-KMT3C / SMYD2 antibody images

  • All lanes : Anti-KMT3C / SMYD2 antibody (ab108217) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH 3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 50 kDa


    Exposure time : 3 minutes
  • All lanes : Anti-KMT3C / SMYD2 antibody (ab108217) at 1/1000 dilution

    Lane 1 : U2OS whole cell lysate - siRNA targeting luciferase
    Lane 2 : U2OS whole cell lysate - siRNA targeting SMYD2
    Lane 3 : U2OS whole cell lysate - siRNA targeting SMYD2

    Lysates/proteins at 15 µg per lane.

    Secondary
    HRP-conjugated goat anti-rabbit IgG at 1/1000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Observed band size : 50 kDa


    Exposure time : 20 seconds

    This image is courtesy of an anonymous Abreview

    Blocked with 5% BSA for 1 hour at room temperature.

    Primary antibody incubation time: 1 hour.

    See Abreview

  • Detection of KMT3C / SMYD2 by Western blot of Immunprecipitate.
    ab108217, at 1 µg/ml, staining KMT3C / SMYD2 in HeLa whole cell lysate immunoprecipitated using ab108217 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with an exposure time of 10 seconds.

References for Anti-KMT3C / SMYD2 antibody (ab108217)

ab108217 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (U2OS cells)
Gel Running Conditions Reduced Denaturing (4-12%)
Loading amount 15 µg
Treatment siLuc and siSMYD2
Specification U2OS cells
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Submitted Mar 23 2015

Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (lung cancer line LKR10 and LKR13)
Specification lung cancer line LKR10 and LKR13
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
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Submitted Jul 25 2014

Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (lung cancer line A549 and H23)
Specification lung cancer line A549 and H23
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
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Submitted Jul 25 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"