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Rabbit polyclonal to KMT3C / SMYD2
Predicted to work with:
Mouse, Rat, Cow, Pig
Recombinant fragment, corresponding to a region within amino acids 115-310 of Human KMT3C/ SMYD2 (UniProt ID: Q9NRG4).
A549, HeLa, HepG2 or HCT116 whole cell lysate; HeLa cells.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Predicted molecular weight: 49 kDa.
1/100 - 1/1000.
Protein-lysine N-methyltransferase that methylates both histones and non-histone proteins. Specifically methylates histone H3 'Lys-4' (H3K4me) and dimethylates histone H3 'Lys-36' (H3K36me2). Has also methyltransferase activity toward non-histone proteins such as p53/TP53 and RB1. Monomethylates 'Lys-370' of p53/TP53, leading to decreased DNA-binding activity and subsequent transcriptional regulation activity of p53/TP53. Monomethylates 'Lys-860' of RB1/RB.
Contains 1 MYND-type zinc finger.
Contains 1 SET domain.
Cytoplasm > cytosol. Nucleus.
Information by UniProt
Histone methyltransferase SMYD2 antibody
HSKM B antibody
Western blot - Anti-KMT3C / SMYD2 antibody (ab154749)
All lanes : Anti-KMT3C / SMYD2 antibody (ab154749) at 1/1000 dilution Lane 1 : HeLa whole cell lysate Lane 2 : HCT116 whole cell lysate Lysates/proteins at 30 µg per lane. Predicted band size: 49 kDa 10% SDS PAGE
Immunocytochemistry/ Immunofluorescence - Anti-KMT3C / SMYD2 antibody (ab154749)
Immunofluorescence analysis of methanol-fixed HeLa cell, labeling KMT3C / SMYD2 using ab154749 at 1/200 dilution. Lower panel was co-stained with Hoechst 33342.
has not yet been referenced specifically in any publications.
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