Synthetic peptide (Human) corresponding to a region between residues 1000 and 1050 of KMT4/ Dot1L. (NP_115871.1).
Our Abpromise guarantee covers the use of ab72454 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|WB||1/2000 - 1/10000. Detects a band of approximately 185 kDa (predicted molecular weight: 185 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|