Overview

  • Product nameAnti-KMT4 / Dot1L antibody
    See all KMT4 / Dot1L primary antibodies
  • Description
    Rabbit polyclonal to KMT4 / Dot1L
  • Tested applicationsSuitable for: IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rhesus monkey, Orangutan
  • Immunogen

    Synthetic peptide (Human) corresponding to a region between residues 1000 and 1050 of KMT4/ Dot1L. (NP_115871.1).

  • Positive control
    • Whole cell HeLa lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab72454 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 185 kDa (predicted molecular weight: 185 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • FunctionHistone methyltransferase. Methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones. Binds to DNA.
  • Sequence similaritiesBelongs to the class I-like SAM-binding methyltransferase superfamily. DOT1 family.
    Contains 1 DOT1 domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Disrupter of telomere silencing protein 1 antibody
    • DOT 1 antibody
    • DOT1 antibody
    • DOT1 like antibody
    • DOT1 like histone H3 methyltransferase (S. cerevisiae) antibody
    • DOT1 like histone H3 methyltransferase antibody
    • DOT1 like histone H3K79 methyltransferase antibody
    • DOT1 like protein antibody
    • DOT1, S. cerevisiae, homolog of antibody
    • DOT1-like protein antibody
    • DOT1L antibody
    • DOT1L_HUMAN antibody
    • EC 2.1.1.43 antibody
    • H3 K79 HMTase antibody
    • H3 lysine-79 specific antibody
    • H3-K79-HMTase antibody
    • Histone H3 K79 methyltransferase antibody
    • Histone H3 methyltransferase antibody
    • Histone H3 methyltransferase DOT1-like antibody
    • Histone H3-K79 methyltransferase antibody
    • Histone lysine N methyltransferase H3 lysine 79 specific antibody
    • Histone methyltransferase DOT1L antibody
    • Histone-lysine N-methyltransferase antibody
    • KIAA1814 antibody
    • KMT4 antibody
    • Lysine N methyltransferase 4 antibody
    • Lysine N-methyltransferase 4 antibody
    see all

Anti-KMT4 / Dot1L antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling KMT4 / Dot1L with ab72454 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-KMT4 / Dot1L antibody (ab72454) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 185 kDa
    Observed band size : 185 kDa
    Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
  • HeLa whole cell lysate ( 1 mg for IP, 20% of IP loaded) with ab72454 at 3 µg/mg lysate for IP, followed by ab72454 at 1 µg/ml for WB.
    Detection: Chemiluminescence with an exposure time of 10 seconds.

References for Anti-KMT4 / Dot1L antibody (ab72454)

This product has been referenced in:
  • Cirillo F  et al. Molecular Mechanisms of Selective Estrogen Receptor Modulator Activity in Human Breast Cancer Cells: Identification of Novel Nuclear Cofactors of Antiestrogen-ERa Complexes by Interaction Proteomics. J Proteome Res : (2012). Read more (PubMed: 23170835) »
  • Benedikt A  et al. The leukemogenic AF4-MLL fusion protein causes P-TEFb kinase activation and altered epigenetic signatures. Leukemia 25:135-44 (2011). WB, IP ; Human . Read more (PubMed: 21030982) »

See all 3 Publications for this product

Product Wall

Thank you for your patience.

I have heard back from the lab and for ab72454, the homology with mouse is, unfortunately,not greater than 50%. Thus, the chance of crossreactivity is quite low and I would not recommend trying this antibody in m...

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Thank you for your reply. The information is very detailed and it is disappointing that the optimization steps did not help improve the results. The additional testing seems to confirm that the product received is not working as expected. The cu...

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