Anti-KMT5A / SETD8 / Pr-SET7 antibody [43AT551.86] (ab3798)


  • Product nameAnti-KMT5A / SETD8 / Pr-SET7 antibody [43AT551.86]
    See all KMT5A / SETD8 / Pr-SET7 primary antibodies
  • Description
    Mouse monoclonal [43AT551.86] to KMT5A / SETD8 / Pr-SET7
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Fusion protein (GST-tag) corresponding to Human KMT5A/ SETD8/ Pr-SET7 (N terminal).

  • Positive control
    • WB: 293, 293T, SAOS and NIH/3T3 cell lysates. IHC-P: Human thyroid gland tissue. FC: HeLa cells.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPBS with 0.1% sodium azide
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesThis antibody is purified through a protein G column, followed by dialysis against PBS.
  • Primary antibody notesSimilar to acetylation and phosphorylation, histone methylation at the N-terminal tail has emerged as an important role in regulating chromatin dynamics and gene activity. Histone methylation occurs on arginine and lysine residues and is catalyzed by two families of proteins, the protein arginine methyltransferase family and the SET-domain-containing methyltransferase family. Five members have been identified in the arginine methyltransferase family. About 27 are grouped into the SET-domain family, and another 17 make up the PR domain family that is related to the SET domain family. The retinoblastoma protein-interacting zinc finger gene RIZ 1 is a tumor suppressor gene and a FOUNDING member of the PR domain family. RIZ 1 inactivation is commonly found in many types of human cancers and occurs through loss of mRNA expression, frame shift mutation, chromosomal deletion, and missense mutation. RIZ 1 is also a tumor susceptibility gene in mice. The loss of RIZ 1 mRNA in human cancers was shown to associate with DNA methylation of its promoter CpG island. Methylation of the RIZ 1 promoter strongly correlated with lost or decreased RIZ 1 mRNA expression in breast, liver, colon, and lung cancer cell lines as well as in liver cancer tissues.
  • ClonalityMonoclonal
  • Clone number43AT551.86
  • IsotypeIgG1
  • Research areas


Our Abpromise guarantee covers the use of ab3798 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/10 - 1/50.
WB 1/1000. Predicted molecular weight: 42.89 kDa.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt Use 1µg for 106 cells.


  • FunctionProtein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones. Mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes.
  • Sequence similaritiesBelongs to the histone-lysine methyltransferase family. PR/SET subfamily.
    Contains 1 SET domain.
  • Developmental stageNot detected during G1 phase. First detected during S through G2 phases, and peaks during mitosis (at protein level).
  • DomainAlthough the SET domain contains the active site of enzymatic activity, both sequences upstream and downstream of the SET domain are required for methyltransferase activity.
  • Cellular localizationNucleus. Chromosome. Specifically localizes to mitotic chromosomes. Associates with silent chromatin on euchromatic arms. Not associated with constitutive heterochromatin.
  • Information by UniProt
  • Database links
  • Alternative names
    • H4 K20 HMTase antibody
    • H4 K20 specific histone methyltransferase antibody
    • H4-K20-HMTase SETD8 antibody
    • Histone H4 K20 methyltransferase antibody
    • Histone lysine N methyltransferase H4 lysine 20 specific antibody
    • Histone-lysine N-methyltransferase SETD8 antibody
    • Lysine N-methyltransferase 5A antibody
    • N-lysine methyltransferase SETD8 antibody
    • Pr SET 7 antibody
    • Pr SET7 antibody
    • PR-Set7 antibody
    • PR/SET domain containing protein 07 antibody
    • PR/SET domain containing protein 8 antibody
    • PR/SET domain-containing protein 07 antibody
    • PR/SET07 antibody
    • PrSET7 antibody
    • SET 07 antibody
    • SET 8 antibody
    • SET domain containing (lysine methyltransferase) 8 antibody
    • SET domain containing 8 antibody
    • SET domain containing lysine methyltransferase 8 antibody
    • SET domain containing protein 8 antibody
    • SET domain-containing protein 8 antibody
    • SET07 antibody
    • SET8 antibody
    • SETD 8 antibody
    • setd8 antibody
    • SETD8_HUMAN antibody
    see all

Anti-KMT5A / SETD8 / Pr-SET7 antibody [43AT551.86] images

  • All lanes : Anti-KMT5A / SETD8 / Pr-SET7 antibody [43AT551.86] (ab3798) at 1/1000 dilution (SET07 Antibody (43AT551.86.76))

    Lane 1 : 293 cell line
    Lane 2 : 293T cell line

    Lysates/proteins at 35 µg per lane.

    Goat anti-mouse IgG H&L (HRP) at 1/3000 dilution

    Predicted band size : 42.89 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)
  • Immunofluoresence was carried out using mouse cells in culture. Conditions: 4% PFA fixation and dilution with 0.5% triton, 10mg/ml BSA in PBS. Dilution of ab3798 used: 1/10. Green = ab3798 detected with secondary antibody (Alexa 488). Blue = DAPI stain.

  • ab3798 (1µg/ml) staining SETD8 in Human thyroid gland using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining of epithelial cells of the thyroid follicle.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

  • Overlay histogram showing HeLa cells stained with ab3798 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab3798, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

References for Anti-KMT5A / SETD8 / Pr-SET7 antibody [43AT551.86] (ab3798)

This product has been referenced in:
  • DeVilbiss AW  et al. Establishing a hematopoietic genetic network through locus-specific integration of chromatin regulators. Proc Natl Acad Sci U S A 110:E3398-407 (2013). Read more (PubMed: 23959865) »
  • Takawa M  et al. Histone lysine methyltransferase SETD8 promotes carcinogenesis by deregulating PCNA expression. Cancer Res 72:3217-27 (2012). Read more (PubMed: 22556262) »

See all 6 Publications for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Mouse Cell lysate - whole cell (fibroblast)
Specification fibroblast
Treatment siRNA
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Aug 06 2013

Thank you for contacting Abcam regarding ab3798. For IHC on paraffin embedded tissues, we recommend a starting dilution of 1ug/ml.  We recommend incubating the tissue for 20 minutes at room temperature. I hope this information is helpful.  Pleas...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HL60)
Loading amount 10 µg
Specification HL60
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jul 18 2007

I'm sorry to hear you are having a problem with ab3798. I would like to suggest the following modifications to your protocol: -the protein is localised in the nucleus, please make sure you use a nuclear extract of your samples -try a 1:100 ...

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