Anti-KMT5A / SETD8 / Pr-SET7 antibody (ab111691)

Overview

  • Product name
    Anti-KMT5A / SETD8 / Pr-SET7 antibody
    See all KMT5A / SETD8 / Pr-SET7 primary antibodies
  • Description
    Rabbit polyclonal to KMT5A / SETD8 / Pr-SET7
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 81-290 of Human KMT5A/ SETD8/ Pr-SET7, isoform 1(42kda) and 2(39KDa)

  • Positive control
    • NT2D1, IMR32, U-87 MG and MCF7 whole cell lysates; HeLa xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab111691 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 39 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones. Mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes.
  • Sequence similarities
    Belongs to the histone-lysine methyltransferase family. PR/SET subfamily.
    Contains 1 SET domain.
  • Developmental stage
    Not detected during G1 phase. First detected during S through G2 phases, and peaks during mitosis (at protein level).
  • Domain
    Although the SET domain contains the active site of enzymatic activity, both sequences upstream and downstream of the SET domain are required for methyltransferase activity.
  • Cellular localization
    Nucleus. Chromosome. Specifically localizes to mitotic chromosomes. Associates with silent chromatin on euchromatic arms. Not associated with constitutive heterochromatin.
  • Information by UniProt
  • Database links
  • Alternative names
    • H4 K20 HMTase antibody
    • H4 K20 specific histone methyltransferase antibody
    • H4-K20-HMTase SETD8 antibody
    • Histone H4 K20 methyltransferase antibody
    • Histone lysine N methyltransferase H4 lysine 20 specific antibody
    • Histone-lysine N-methyltransferase SETD8 antibody
    • Lysine N-methyltransferase 5A antibody
    • N-lysine methyltransferase SETD8 antibody
    • Pr SET 7 antibody
    • Pr SET7 antibody
    • PR-Set7 antibody
    • PR/SET domain containing protein 07 antibody
    • PR/SET domain containing protein 8 antibody
    • PR/SET domain-containing protein 07 antibody
    • PR/SET07 antibody
    • PrSET7 antibody
    • SET 07 antibody
    • SET 8 antibody
    • SET domain containing (lysine methyltransferase) 8 antibody
    • SET domain containing 8 antibody
    • SET domain containing lysine methyltransferase 8 antibody
    • SET domain containing protein 8 antibody
    • SET domain-containing protein 8 antibody
    • SET07 antibody
    • SET8 antibody
    • SETD 8 antibody
    • setd8 antibody
    • SETD8_HUMAN antibody
    see all

Images

  • Anti-KMT5A / SETD8 / Pr-SET7 antibody (ab111691) at 1/2000 dilution + U-87 MG whole cell lysate at 30 µg

    Predicted band size : 39 kDa
  • ab111691 at 1/500 dilution staining KMT5A / SETD8 / Pr-SET7 in Formalin-fixed, paraffin-embedded HeLa xenograft tissue by Immunohistochemistry.

References

ab111691 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Mouse Cell lysate - whole cell (fibroblast)
Specification
fibroblast
Treatment
siRNA for 48hrs
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

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Verified customer

Submitted Jul 09 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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