Recombinant
RabMAb

Anti-KMT5A / SETD8 / Pr-SET7 antibody [EPR11322] (ab157462)

Overview

  • Product name
    Anti-KMT5A / SETD8 / Pr-SET7 antibody [EPR11322]
    See all KMT5A / SETD8 / Pr-SET7 primary antibodies
  • Description
    Rabbit monoclonal [EPR11322] to KMT5A / SETD8 / Pr-SET7
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human KMT5A/ SETD8/ Pr-SET7 (UniProt: Q9NQR1).

  • Positive control
    • 293T and Saos-2 cell lysates; Permeabilized 293T cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab157462 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function
      Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones. Mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes.
    • Sequence similarities
      Belongs to the histone-lysine methyltransferase family. PR/SET subfamily.
      Contains 1 SET domain.
    • Developmental stage
      Not detected during G1 phase. First detected during S through G2 phases, and peaks during mitosis (at protein level).
    • Domain
      Although the SET domain contains the active site of enzymatic activity, both sequences upstream and downstream of the SET domain are required for methyltransferase activity.
    • Cellular localization
      Nucleus. Chromosome. Specifically localizes to mitotic chromosomes. Associates with silent chromatin on euchromatic arms. Not associated with constitutive heterochromatin.
    • Information by UniProt
    • Database links
    • Alternative names
      • H4 K20 HMTase antibody
      • H4 K20 specific histone methyltransferase antibody
      • H4-K20-HMTase SETD8 antibody
      • Histone H4 K20 methyltransferase antibody
      • Histone lysine N methyltransferase H4 lysine 20 specific antibody
      • Histone-lysine N-methyltransferase SETD8 antibody
      • Lysine N-methyltransferase 5A antibody
      • N-lysine methyltransferase SETD8 antibody
      • Pr SET 7 antibody
      • Pr SET7 antibody
      • PR-Set7 antibody
      • PR/SET domain containing protein 07 antibody
      • PR/SET domain containing protein 8 antibody
      • PR/SET domain-containing protein 07 antibody
      • PR/SET07 antibody
      • PrSET7 antibody
      • SET 07 antibody
      • SET 8 antibody
      • SET domain containing (lysine methyltransferase) 8 antibody
      • SET domain containing 8 antibody
      • SET domain containing lysine methyltransferase 8 antibody
      • SET domain containing protein 8 antibody
      • SET domain-containing protein 8 antibody
      • SET07 antibody
      • SET8 antibody
      • SETD 8 antibody
      • setd8 antibody
      • SETD8_HUMAN antibody
      see all

    Images

    • All lanes : Anti-KMT5A / SETD8 / Pr-SET7 antibody [EPR11322] (ab157462) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : Saos-2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 42 kDa
    • Flow cytometric analysis of permeabilized 293T cells labeling KMT5A / SETD8 / Pr-SET7 with ab157462 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

    References

    ab157462 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Mouse Cell lysate - whole cell (fibroblast)
    Specification
    fibroblast
    Treatment
    siRNA
    Blocking step
    Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 26 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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