Overview

  • Product name
    Anti-KMT5C/SUV4-20h2 antibody
  • Description
    Rabbit polyclonal to KMT5C/SUV4-20h2
  • Tested applications
    Suitable for: WB, ELISA, ChIPmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide from the center region of Human KMT5C/SUV4-20h2 conjugated to KLH

  • Positive control
    • Mouse stomach tissue lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Ammonium Sulphate Precipitation
  • Purification notes
    Following purification, this antibody was dialysed against PBS
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab91224 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Predicted molecular weight: 52 kDa.
ELISA 1/1000.
ChIP Use at an assay dependent concentration. PubMed: 24459145

Target

  • Function
    Histone methyltransferase that specifically trimethylates 'Lys-20' of histone H4. H4 'Lys-20' trimethylation represents a specific tag for epigenetic transcriptional repression. Mainly functions in pericentric heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin in these regions. SUV420H1 is targeted to histone H3 via its interaction with RB1 family proteins (RB1, RBL1 and RBL2).
  • Sequence similarities
    Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar4-20 subfamily.
    Contains 1 SET domain.
  • Cellular localization
    Nucleus. Chromosome. Associated with pericentric heterochromatin. CBX1 and CBX5 are required for the localization to pericentric heterochromatin.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ98627 antibody
    • Histone lysine N methyltransferase SUV420H2 antibody
    • Histone-lysine N-methyltransferase SUV420H2 antibody
    • KMT5C antibody
    • Lysine N methyltransferase 5C antibody
    • Lysine N-methyltransferase 5C antibody
    • MGC2705 antibody
    • Su(var)4 20 homolog 2 antibody
    • Su(var)4-20 homolog 2 antibody
    • Suppressor of variegation 4 20 homolog 2 antibody
    • Suppressor of variegation 4-20 homolog 2 antibody
    • Suv4 20h2 antibody
    • Suv4-20h2 antibody
    • SUV420H2 antibody
    • SV422_HUMAN antibody
    see all

Images

  • Anti-KMT5C/SUV4-20h2 antibody (ab91224) at 1/50 dilution + Mouse stomach tissue lysate at 35 µg

    Predicted band size : 52 kDa
  • Chromatin from cells was immunoprecipitated with IgG (negative control) or SUV420H2 antibodies (ab91224), and DNA was extracted from the immunoprecipitated samples. Immunoprecipitated DNA was subjected to real-time PCR with primer sets that span the TMS1 genomic regions and is expressed as a percentage of input DNA. Data are mean ± S.D. from a representative experiment from triplicate determinants.

References

This product has been referenced in:
  • Nelson DM  et al. Mapping H4K20me3 onto the chromatin landscape of senescent cells indicates a function in control of cell senescence and tumor suppression through preservation of genetic and epigenetic stability. Genome Biol 17:158 (2016). WB ; Human . Read more (PubMed: 27457071) »
  • Hatanaka Y  et al. Histone chaperone CAF-1 mediates repressive histone modifications to protect preimplantation mouse embryos from endogenous retrotransposons. Proc Natl Acad Sci U S A 112:14641-6 (2015). ICC/IF ; Mouse . Read more (PubMed: 26546670) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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