Overview

  • Product nameAnti-KMT5C/SUV4-20h2 antibody - ChIP Grade
  • Description
    Rabbit polyclonal to KMT5C/SUV4-20h2 - ChIP Grade
  • Tested applicationsSuitable for: WB, ELISA, ChIPmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide from the center region of Human KMT5C/SUV4-20h2 conjugated to KLH

  • Positive control
    • Mouse stomach tissue lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab91224 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Predicted molecular weight: 52 kDa.
ELISA 1/1000.
ChIP Use at an assay dependent concentration. PubMed: 24459145

Target

  • FunctionHistone methyltransferase that specifically trimethylates 'Lys-20' of histone H4. H4 'Lys-20' trimethylation represents a specific tag for epigenetic transcriptional repression. Mainly functions in pericentric heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin in these regions. SUV420H1 is targeted to histone H3 via its interaction with RB1 family proteins (RB1, RBL1 and RBL2).
  • Sequence similaritiesBelongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar4-20 subfamily.
    Contains 1 SET domain.
  • Cellular localizationNucleus. Chromosome. Associated with pericentric heterochromatin. CBX1 and CBX5 are required for the localization to pericentric heterochromatin.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ98627 antibody
    • Histone lysine N methyltransferase SUV420H2 antibody
    • Histone-lysine N-methyltransferase SUV420H2 antibody
    • KMT5C antibody
    • Lysine N methyltransferase 5C antibody
    • Lysine N-methyltransferase 5C antibody
    • MGC2705 antibody
    • Su(var)4 20 homolog 2 antibody
    • Su(var)4-20 homolog 2 antibody
    • Suppressor of variegation 4 20 homolog 2 antibody
    • Suppressor of variegation 4-20 homolog 2 antibody
    • Suv4 20h2 antibody
    • Suv4-20h2 antibody
    • SUV420H2 antibody
    • SV422_HUMAN antibody
    see all

Anti-KMT5C/SUV4-20h2 antibody - ChIP Grade images

  • Anti-KMT5C/SUV4-20h2 antibody - ChIP Grade (ab91224) at 1/50 dilution + Mouse stomach tissue lysate at 35 µg

    Predicted band size : 52 kDa
  • Chromatin from cells was immunoprecipitated with IgG (negative control) or SUV420H2 antibodies (ab91224), and DNA was extracted from the immunoprecipitated samples. Immunoprecipitated DNA was subjected to real-time PCR with primer sets that span the TMS1 genomic regions and is expressed as a percentage of input DNA. Data are mean ± S.D. from a representative experiment from triplicate determinants.

References for Anti-KMT5C/SUV4-20h2 antibody - ChIP Grade (ab91224)

This product has been referenced in:
  • Kapoor-Vazirani P & Vertino PM A dual role for the histone methyltransferase PR-SET7/SETD8 and histone H4 lysine 20 monomethylation in the local regulation of RNA polymerase II pausing. J Biol Chem 289:7425-37 (2014). ChIP . Read more (PubMed: 24459145) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"