The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100. Predicted molecular weight: 52 kDa.
Use at an assay dependent concentration. PubMed: 24459145
FunctionHistone methyltransferase that specifically trimethylates 'Lys-20' of histone H4. H4 'Lys-20' trimethylation represents a specific tag for epigenetic transcriptional repression. Mainly functions in pericentric heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin in these regions. SUV420H1 is targeted to histone H3 via its interaction with RB1 family proteins (RB1, RBL1 and RBL2).
Sequence similaritiesBelongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar4-20 subfamily. Contains 1 SET domain.
Cellular localizationNucleus. Chromosome. Associated with pericentric heterochromatin. CBX1 and CBX5 are required for the localization to pericentric heterochromatin.
Anti-KMT5C/SUV4-20h2 antibody - ChIP Grade (ab91224) at 1/50 dilution + Mouse stomach tissue lysate at 35 µg
Predicted band size : 52 kDa
ChIP - Anti-KMT5C/SUV4-20h2 antibody - ChIP Grade (ab91224)Image from Kapoor-Vazirani P et al., J Biol Chem. 2014;289(11):7425-37. Fig 5.; doi: 10.1074/jbc.M113.520783.
Chromatin from cells was immunoprecipitated with IgG (negative control) or SUV420H2 antibodies (ab91224), and DNA was extracted from the immunoprecipitated samples. Immunoprecipitated DNA was subjected to real-time PCR with primer sets that span the TMS1 genomic regions and is expressed as a percentage of input DNA. Data are mean ± S.D. from a representative experiment from triplicate determinants.
References for Anti-KMT5C/SUV4-20h2 antibody - ChIP Grade (ab91224)
This product has been referenced in:
Nelson DM et al. Mapping H4K20me3 onto the chromatin landscape of senescent cells indicates a function in control of cell senescence and tumor suppression through preservation of genetic and epigenetic stability. Genome Biol17:158 (2016).
Read more (PubMed: 27457071) »
Hatanaka Y et al. Histone chaperone CAF-1 mediates repressive histone modifications to protect preimplantation mouse embryos from endogenous retrotransposons. Proc Natl Acad Sci U S A112:14641-6 (2015).
Read more (PubMed: 26546670) »