Overview

  • Product nameAnti-KMT8/Riz1/Riz2 antibody
  • Description
    Rabbit polyclonal to KMT8/Riz1/Riz2
  • SpecificityThis antibody recognizes the beta isoform (Riz 2) of PRDM 2 (185 kDa) as well as RIZ 1. It will not detect isoform 4.
  • Tested applicationsIP, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Purified recombinant fusion protein, corresponding to amino acids 245-573 of Rat PRDM 2.

  • Positive control
    • Rat B50 brain tumour cell lysate.
  • General notes


    Similar to acetylation and phosphorylation, histone methylation at the N-terminal tail has emerged as an important role in regulating chromatin dynamics and gene activity. Histone methylation occurs on arginine and lysine residues and is catalyzed by two families of proteins, the protein arginine methyltransferase family and the SET-domain-containing methyltransferase family. Five members have been identified in the arginine methyltransferase family. About 27 are grouped into the SET-domain family, and another 17 make up the PR domain family that is related to the SET domain family. The retinoblastoma protein-interacting zinc finger gene RIZ 1 is a tumor suppressor gene and a founding member of the PR domain family. RIZ 1 inactivation is commonly found in many types of human cancers and occurs through loss of mRNA expression, frame shift mutation, chromosomal deletion, and missense mutation. RIZ 1 is also a tumor susceptibility gene in mice. The loss of RIZ 1 mRNA in human cancers was shown to associate with DNA methylation of its promoter CpG island. Methylation of the RIZ 1 promoter strongly correlated with lost or decreased RIZ 1 mRNA expression in breast, liver, colon, and lung cancer cell lines as well as in liver cancer tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab3790 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/100.
WB 1/100 - 1/500. Predicted molecular weight: 189 kDa.
ELISA 1/1000.

Target

  • FunctionS-adenosyl-L-methionine-dependent histone methyltransferase that specifically methylates 'Lys-9' of histone H3. May function as a DNA-binding transcription factor. Binds to the macrophage-specific TPA-responsive element (MTE) of the HMOX1 (heme oxygenase 1) gene and may act as a transcriptional activator of this gene.
  • Tissue specificityHighly expressed in retinoblastoma cell lines and in brain tumors. Also expressed in a number of other cell lines and in brain, heart, skeletal muscle, liver and spleen. Isoform 1 is expressed in testis at much higher level than isoform 3.
  • Sequence similaritiesContains 8 C2H2-type zinc fingers.
    Contains 1 SET domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • GATA 3 binding protein G3B antibody
    • GATA-3-binding protein G3B antibody
    • HUMHOXY 1 antibody
    • HUMHOXY1 antibody
    • HUMHOXY1 antibody
    • Lysine N methyltransferase 8 antibody
    • Lysine N-methyltransferase 8 antibody
    • MTB ZF antibody
    • MTB-ZF antibody
    • MTE binding protein antibody
    • MTE-binding protein antibody
    • PR domain containing 2 with ZNF domain antibody
    • PR domain containing 2 with ZNF domain antibody
    • PR domain containing protein 2 antibody
    • PR domain zinc finger protein 2 antibody
    • PR domain-containing protein 2 antibody
    • PR-domain zinc finger protein 2 antibody
    • PRDM 2 antibody
    • PRDM2 antibody
    • PRDM2_HUMAN antibody
    • Retinoblastoma protein binding zinc finger protein antibody
    • Retinoblastoma protein interacting zinc- inger protein antibody
    • retinoblastoma protein-interacting zinc finger protein antibody
    • retinoblastoma protein-interacting zinc finger protein antibody
    • Retinoblastoma protein-interacting zinc-finger protein antibody
    • RIZ 1 antibody
    • RIZ 2 antibody
    • RIZ antibody
    • RIZ antibody
    • RIZ-LSB antibody
    • RIZ-LSB antibody
    • RIZ1 antibody
    • RIZ1 antibody
    • RIZ2 antibody
    • RIZ2 antibody
    • Zinc finger DNA binding protein antibody
    • Zinc finger protein RIZ antibody
    see all

Anti-KMT8/Riz1/Riz2 antibody images

  • A) Rat B50 brain tumor cell extracts were immunoprecipitated with RIZ antibody or preimmune (Liu L. et al. J. Biol. Chem. 1997. 272: 2984.).

    B) Immunohistochemical analysis of RIZ1 in adenovirus-transduced tumor cells. Established HCT116 xenograft tumors at day 2 after infection of AdRIZ1, Adnull, or buffer alone were processed for RIZ1 in situ detection (TUNEL) analysis (Jiang G-L. and Huang S. Cancer Res. 2001. 61: 1796.)

References for Anti-KMT8/Riz1/Riz2 antibody (ab3790)

This product has been referenced in:
  • Congdon LM  et al. The PR-Set7 binding domain of Riz1 is required for the H4K20me1-H3K9me1 trans-tail 'histone code' and Riz1 tumor suppressor function. Nucleic Acids Res 42:3580-9 (2014). WB ; Human . Read more (PubMed: 24423864) »
  • Zhou W  et al. Requirement of RIZ1 for cancer prevention by methyl-balanced diet. PLoS ONE 3:e3390 (2008). WB ; Mouse . Read more (PubMed: 18852888) »

See all 2 Publications for this product

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