Overview

  • Product nameAnti-KMT8/Riz1/Riz2 antibody
  • Description
    Rabbit polyclonal to KMT8/Riz1/Riz2
  • SpecificityThis antibody recognizes the beta isoform (Riz 2) of PRDM 2 (185 kDa) as well as RIZ 1. It will not detect isoform 4.
  • Tested applicationsIP, WB, IHC, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Fusion protein (GST-tag) corresponding to Rat KMT8/Riz1/Riz2 aa 245-573.

  • Positive control
    • IP: Rat B50 brain tumour cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab3790 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 189 kDa. PubMed: 24423864
IHC Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P 1/100 - 1/500.

Target

  • FunctionS-adenosyl-L-methionine-dependent histone methyltransferase that specifically methylates 'Lys-9' of histone H3. May function as a DNA-binding transcription factor. Binds to the macrophage-specific TPA-responsive element (MTE) of the HMOX1 (heme oxygenase 1) gene and may act as a transcriptional activator of this gene.
  • Tissue specificityHighly expressed in retinoblastoma cell lines and in brain tumors. Also expressed in a number of other cell lines and in brain, heart, skeletal muscle, liver and spleen. Isoform 1 is expressed in testis at much higher level than isoform 3.
  • Sequence similaritiesContains 8 C2H2-type zinc fingers.
    Contains 1 SET domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • GATA 3 binding protein G3B antibody
    • GATA-3-binding protein G3B antibody
    • HUMHOXY 1 antibody
    • HUMHOXY1 antibody
    • HUMHOXY1 antibody
    • Lysine N methyltransferase 8 antibody
    • Lysine N-methyltransferase 8 antibody
    • MTB ZF antibody
    • MTB-ZF antibody
    • MTE binding protein antibody
    • MTE-binding protein antibody
    • PR domain containing 2 with ZNF domain antibody
    • PR domain containing 2 with ZNF domain antibody
    • PR domain containing protein 2 antibody
    • PR domain zinc finger protein 2 antibody
    • PR domain-containing protein 2 antibody
    • PR-domain zinc finger protein 2 antibody
    • PRDM 2 antibody
    • PRDM2 antibody
    • PRDM2_HUMAN antibody
    • Retinoblastoma protein binding zinc finger protein antibody
    • Retinoblastoma protein interacting zinc- inger protein antibody
    • retinoblastoma protein-interacting zinc finger protein antibody
    • retinoblastoma protein-interacting zinc finger protein antibody
    • Retinoblastoma protein-interacting zinc-finger protein antibody
    • RIZ 1 antibody
    • RIZ 2 antibody
    • RIZ antibody
    • RIZ antibody
    • RIZ-LSB antibody
    • RIZ-LSB antibody
    • RIZ1 antibody
    • RIZ1 antibody
    • RIZ2 antibody
    • RIZ2 antibody
    • Zinc finger DNA binding protein antibody
    • Zinc finger protein RIZ antibody
    see all

Anti-KMT8/Riz1/Riz2 antibody images

  • Rat B50 brain tumor cell extracts were immunoprecipitated with RIZ antibody or preimmune (Liu L. et al. J. Biol. Chem. 1997. 272: 2984.).

  • Immunohistochemical analysis of RIZ1 in adenovirus-transduced tumor cells. Established HCT116 xenograft tumors at day 2 after infection of AdRIZ1, Adnull, or buffer alone were processed for RIZ1 in situ detection (TUNEL) analysis (Jiang G-L. and Huang S. Cancer Res. 2001. 61: 1796.)

References for Anti-KMT8/Riz1/Riz2 antibody (ab3790)

This product has been referenced in:
  • Congdon LM  et al. The PR-Set7 binding domain of Riz1 is required for the H4K20me1-H3K9me1 trans-tail 'histone code' and Riz1 tumor suppressor function. Nucleic Acids Res 42:3580-9 (2014). WB ; Human . Read more (PubMed: 24423864) »
  • Zhou W  et al. Requirement of RIZ1 for cancer prevention by methyl-balanced diet. PLoS ONE 3:e3390 (2008). WB ; Mouse . Read more (PubMed: 18852888) »

See all 2 Publications for this product

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