Overview

  • Product nameKN-93 (water soluble)
  • Description
    Potent, specific CaMK II inhibitor
  • Biological descriptionPotent, specific and cell-permeable calmodulin-dependent protein kinase (CaMK) II inhibitor (IC50 = 370 nM). Also a potent and direct extracellular open channel voltage-gated potassium channel blocker (IC50 = 307 nM for Kv1.5); independent of CaM kinase II inhibition. Water soluble.
  • Purity> 99%

Properties

    Applications

    Our Abpromise guarantee covers the use of ab120980 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.

    KN-93 (water soluble) images

    • ab40754 staining cyclin D1 in MCF7 cells treated with KN-93 (water soluble) (ab120980), by ICC/IF. Decrease in cyclin D1 expression correlates with increased concentration of KN-93 (water soluble), as described in literature.
      The cells were incubated at 37°C for 24 hours in media containing different concentrations of ab120980 (KN-93 (water soluble)) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab40754 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

    References for KN-93 (water soluble) (ab120980)

    This product has been referenced in:
    • Ke J  et al. Effects of calmodulin-dependent protein kinase II inhibitor, KN-93, on electrophysiological features of rabbit hypertrophic cardiac myocytes. J Huazhong Univ Sci Technolog Med Sci 32:485-9 (2012). Read more (PubMed: 22886958) »
    • Rezazadeh S  et al. KN-93 (2-[N-(2-hydroxyethyl)]-N-(4-methoxybenzenesulfonyl)]amino-N-(4-chlorocinnamyl)-N-methylbenzylamine), a calcium/calmodulin-dependent protein kinase II inhibitor, is a direct extracellular blocker of voltage-gated potassium channels. J Pharmacol Exp Ther 317:292-9 (2006). Read more (PubMed: 16368898) »
    • Sumi M  et al. The newly synthesized selective Ca2+/calmodulin dependent protein kinase II inhibitor KN-93 reduces dopamine contents in PC12h cells. Biochem Biophys Res Commun 181:968-75 (1991). Read more (PubMed: 1662507) »

    See 1 Publication for this product

    Product Wall

    The lab have completed our analysis and can confirm that this product is soluble in water to 100 mM with prolonged shaking.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"