Anti-KPC2 antibody (ab151317)
Key features and details
- Rabbit polyclonal to KPC2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-KPC2 antibody
See all KPC2 primary antibodies -
Description
Rabbit polyclonal to KPC2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
A recombinant protein fragment corresponding to a region within amino acids 69 and 348 of Human KPC2 (UniProt ID: Q9BSL1).
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Positive control
- A549; HepG2 and HCT116 cell lysates; HeLa cells; Human hepatoma tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151317 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 45 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 45 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
Target
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Relevance
KPC2 (Kip1 ubiquitylation-promoting complex 2) together with KPC1 forms the ubiquitin ligase KPC, which regulates degradation of the cyclin-dependent kinase inhibitor p27 at the G1 phase of the cell cycle. KPC2 contains a ubiquitin-like (UBL) domain, two ubiquitin-associated (UBA) domains, and a heat shock chaperonin-binding (STI1) domain. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 10422 Human
- Omim: 608129 Human
- SwissProt: Q9BSL1 Human
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Alternative names
- 1110033G07Rik antibody
- AA407978 antibody
- E3 ubiquitin-protein ligase subunit KPC2 antibody
see all
Images
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Anti-KPC2 antibody (ab151317) at 1/1000 dilution + A549 cell lysate at 30 µg
Predicted band size: 45 kDa
10% SDS PAGE -
Immunohistochemical analysis of paraffin-embedded Human hepatoma labeling KPC2 using ab151317 at 1/500 dilution.
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Immunofluorescence analysis of methanol-fixed HeLa cells, labeling KPC2 using ab151317 at 1/200 dilution. Right panel costained with Hoechst 33342.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab151317 has been referenced in 1 publication.
- Zhuang K et al. Gastrin induces multidrug resistance via the degradation of p27Kip1 in the gastric carcinoma cell line SGC7901. Int J Oncol 50:2091-2100 (2017). PubMed: 28498440