Overview

  • Product name
  • Description
    Rabbit polyclonal to KPC2
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Zebrafish
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human KPC2.

  • Positive control
    • This antibody gave a positive signal in Human Liver Tissue Lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab61596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 45 kDa).

Target

  • Relevance
    KPC2 (Kip1 ubiquitylation-promoting complex 2) together with KPC1 forms the ubiquitin ligase KPC, which regulates degradation of the cyclin-dependent kinase inhibitor p27 at the G1 phase of the cell cycle. KPC2 contains a ubiquitin-like (UBL) domain, two ubiquitin-associated (UBA) domains, and a heat shock chaperonin-binding (STI1) domain.
  • Cellular localization
    Cytoplasmic
  • Database links
  • Alternative names
    • 1110033G07Rik antibody
    • AA407978 antibody
    • E3 ubiquitin-protein ligase subunit KPC2 antibody
    • GBDR1 antibody
    • Glialblastoma cell differentiation-related protein 1 antibody
    • Glialblastoma differentiation-related protein 1 antibody
    • Kip1 ubiquitination-promoting complex protein 2 antibody
    • kpc 2 antibody
    • Kpc2 antibody
    • OTTHUMP00000022563 antibody
    • Putative glialblastoma cell differentiation-related protein antibody
    • RP11-432J22.3 antibody
    • UBA domain containing 1 antibody
    • UBA domain-containing protein 1 antibody
    • UBAC 1 antibody
    • UBAC1 antibody
    • Ubadc1 antibody
    • Ubiquitin associated domain containing 1 antibody
    • Ubiquitin-associated domain-containing protein 1 antibody
    see all

Images

  • Anti-KPC2 antibody (ab61596) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution

    Predicted band size : 45 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 15 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

References

ab61596 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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