Overview

  • Product nameAnti-KPNA2 antibody
    See all KPNA2 primary antibodies
  • Description
    Rabbit polyclonal to KPNA2
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region within C terminal amino acids 475-529 (ALQNHENESV YKASLSLIEK YFSVEEEEDQ NVVPETTSEG YTFQVQDGAP GTFNF) of human KPNA2 (NP_002257.1).

  • Positive control
    • Human: metastatic lymph node, bladder carcinoma. Mouse: squamous cell carcinoma, teratoma.
  • General notes

    Concentration is optimized for IHC and not determined

Properties

Applications

Our Abpromise guarantee covers the use of ab84440 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Use under non reducing condition. Predicted molecular weight: 58 kDa.
IHC-P 1/100 - 1/500.

Target

  • FunctionFunctions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.
  • Tissue specificityExpressed ubiquitously.
  • Sequence similaritiesBelongs to the importin alpha family.
    Contains 10 ARM repeats.
    Contains 1 IBB domain.
  • DomainConsists of an N-terminal hydrophilic region, a hydrophobic central region composed of 10 repeats, and a short hydrophilic C-terminus. The N-terminal hydrophilic region contains the importin beta binding domain (IBB domain), which is sufficient for binding importin beta and essential for nuclear protein import.
    The IBB domain is thought to act as an intrasteric autoregulatory sequence by interacting with the internal autoinhibitory NLS. Binding of KPNB1 probably overlaps the internal NLS and contributes to a high affinity for cytoplasmic NLS-containing cargo substrates. After dissociation of the importin/substrate complex in the nucleus the internal autohibitory NLS contributes to a low affinity for nuclear NLS-containing proteins.
    The major and minor NLS binding sites are mainly involved in recognition of simple or bipartite NLS motifs. Structurally located within in a helical surface groove they contain several conserved Trp and Asn residues of the corresponding third helices (H3) of ARM repeats which mainly contribute to binding.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • IMA1_HUMAN antibody
    • Importin alpha 1 antibody
    • Importin alpha 2 antibody
    • Importin alpha 2 subunit antibody
    • Importin alpha P1 antibody
    • Importin subunit alpha-1 antibody
    • IPO A1 antibody
    • IPOA 1 antibody
    • IPOA1 antibody
    • Karyopherin alpha 2 antibody
    • Karyopherin alpha 2 RAG cohort 1 antibody
    • Karyopherin alpha 2 subunit antibody
    • Karyopherin subunit alpha-2 antibody
    • KPNA2 antibody
    • KPNA2 protein antibody
    • Pendulin antibody
    • QIP 2 antibody
    • QIP2 antibody
    • RAG cohort 1 antibody
    • RAG cohort protein 1 antibody
    • RCH 1 antibody
    • RCH1 antibody
    • SRP 1 antibody
    • SRP1 alpha antibody
    • SRP1 antibody
    • SRP1-alpha antibody
    • SRP1alpha antibody
    see all

Anti-KPNA2 antibody images

  • Immunohistochemical analysis of KPNA2 in formalin fixed, paraffin embedded human metastatic lymph node tissue, using a 1/250 dilution of ab84440.
    Detection: DAB staining.
  • Immunohistochemical analysis of KPNA2 in formalin fixed, paraffin embedded human bladder carcinoma tissue, using a 1/250 dilution of ab84440.
    Detection: DAB staining.
  • Immunohistochemical analysis of KPNA2 in formalin fixed, paraffin embedded mouse squamous cell carcinoma tissue, using a 1/250 dilution of ab84440.
    Detection: DAB staining.
  • Immunohistochemical analysis of KPNA2 in formalin fixed, paraffin embedded mouse teratoma tissue, using a 1/250 dilution of ab84440.
    Detection: DAB staining.
  • Immunohistochemical analysis of Human ovarian germ cell tumour, staining KPNA2 with ab84440.

    Antigen retrieval was performed by submerging the sample in citrate buffer (pH 6) and microwaving. The sections were blocked with 1% BSA before incubating with primary antibody (1/400). Staining was detected using DAB.

  • Developed using the ECL technique

    Performed under non-reducing conditions.

    Predicted band size : 58 kDa
    Observed band size : 58 kDa


    Exposure time : 3 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-KPNA2 antibody (ab84440)

This product has been referenced in:
  • Yang CP  et al. Identification and characterization of nuclear and nucleolar localization signals in 58-kDa microspherule protein (MSP58). J Biomed Sci 22:33 (2015). WB ; Human . Read more (PubMed: 25981436) »
  • Rachidi SM  et al. Molecular profiling of multiple human cancers defines an inflammatory cancer-associated molecular pattern and uncovers KPNA2 as a uniform poor prognostic cancer marker. PLoS One 8:e57911 (2013). IHC-P ; Human . Read more (PubMed: 23536776) »

See all 4 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Day28 Testis)
Loading amount 75 µg
Specification Day28 Testis
Gel Running Conditions Non-reduced Denaturing (12% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted Nov 08 2012

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