Overview

  • Product nameAnti-KPNA3 antibody
    See all KPNA3 primary antibodies
  • Description
    Rabbit polyclonal to KPNA3
  • Specificityab105348 is predicted not to cross-react with other Importin alpha family members.
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A 14 amino acid synthetic peptide from near the carboxy end of Human KPNA3 (NP_002258).

  • Positive control
    • EL4 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab105348 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 58 kDa.

Target

  • FunctionFunctions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS. Recognizes NLSs of influenza A virus nucleoprotein probably through ARM repeats 7-9.
  • Tissue specificityUbiquitous. Highest levels in heart and skeletal muscle.
  • Sequence similaritiesBelongs to the importin alpha family.
    Contains 10 ARM repeats.
    Contains 1 IBB domain.
  • DomainConsists of an N-terminal hydrophilic region, a hydrophobic central region composed of 10 repeats, and a short hydrophilic C-terminus. The N-terminal hydrophilic region contains the importin beta binding domain (IBB domain), which is sufficient for binding importin beta and essential for nuclear protein import.
    The IBB domain is thought to act as an intrasteric autoregulatory sequence by interacting with the internal autoinhibitory NLS. Binding of KPNB1 probably overlaps the internal NLS and contributes to a high affinity for cytoplasmic NLS-containing cargo substrates. After dissociation of the importin/substrate complex in the nucleus the internal autohibitory NLS contributes to a low affinity for nuclear NLS-containing proteins.
    The major and minor NLS binding sites are mainly involved in recognition of simple or bipartite NLS motifs. Structurally located within in a helical surface groove they contain several conserved Trp and Asn residues of the corresponding third helices (H3) of ARM repeats which mainly contribute to binding.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • SRP1 gamma antibody
    • hSRP1 antibody
    • IMA4_HUMAN antibody
    • Importin alpha 3 antibody
    • Importin alpha 3 subunit antibody
    • Importin alpha 4 antibody
    • Importin alpha Q2 antibody
    • Importin subunit alpha-4 antibody
    • IPOA4 antibody
    • Karyopherin alpha 3 antibody
    • Karyopherin alpha 3 importin alpha 4 antibody
    • Karyopherin alpha 3 subunit antibody
    • Karyopherin subunit alpha 3 antibody
    • Karyopherin subunit alpha-3 antibody
    • KPNA 3 antibody
    • Kpna3 antibody
    • Qip2 antibody
    • SRP1 antibody
    • SRP1-gamma antibody
    • SRP1gamma antibody
    • SRP4 antibody
    see all

Anti-KPNA3 antibody images

  • Lane 1 : Anti-KPNA3 antibody (ab105348) at 1 µg/ml
    Lane 2 : Anti-KPNA3 antibody (ab105348) at 2 µg/ml

    Lane 1 : EL4 cell lysate
    Lane 2 : EL4 cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 58 kDa

References for Anti-KPNA3 antibody (ab105348)

ab105348 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"