• Product nameAnti-KPNA3 antibody
    See all KPNA3 primary antibodies
  • Description
    Rabbit polyclonal to KPNA3
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide conjugated to KLH, from the C terminal region of Human KPNA3 (NP_002258.2).

  • Positive control
    • Human Adrenal, Brain (Cerebellum) and Human Colon tissues.



Our Abpromise guarantee covers the use of ab117578 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 57 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • FunctionFunctions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS. Recognizes NLSs of influenza A virus nucleoprotein probably through ARM repeats 7-9.
  • Tissue specificityUbiquitous. Highest levels in heart and skeletal muscle.
  • Sequence similaritiesBelongs to the importin alpha family.
    Contains 10 ARM repeats.
    Contains 1 IBB domain.
  • DomainConsists of an N-terminal hydrophilic region, a hydrophobic central region composed of 10 repeats, and a short hydrophilic C-terminus. The N-terminal hydrophilic region contains the importin beta binding domain (IBB domain), which is sufficient for binding importin beta and essential for nuclear protein import.
    The IBB domain is thought to act as an intrasteric autoregulatory sequence by interacting with the internal autoinhibitory NLS. Binding of KPNB1 probably overlaps the internal NLS and contributes to a high affinity for cytoplasmic NLS-containing cargo substrates. After dissociation of the importin/substrate complex in the nucleus the internal autohibitory NLS contributes to a low affinity for nuclear NLS-containing proteins.
    The major and minor NLS binding sites are mainly involved in recognition of simple or bipartite NLS motifs. Structurally located within in a helical surface groove they contain several conserved Trp and Asn residues of the corresponding third helices (H3) of ARM repeats which mainly contribute to binding.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • SRP1 gamma antibody
    • hSRP1 antibody
    • IMA4_HUMAN antibody
    • Importin alpha 3 antibody
    • Importin alpha 3 subunit antibody
    • Importin alpha 4 antibody
    • Importin alpha Q2 antibody
    • Importin subunit alpha-4 antibody
    • IPOA4 antibody
    • Karyopherin alpha 3 antibody
    • Karyopherin alpha 3 importin alpha 4 antibody
    • Karyopherin alpha 3 subunit antibody
    • Karyopherin subunit alpha 3 antibody
    • Karyopherin subunit alpha-3 antibody
    • KPNA 3 antibody
    • Kpna3 antibody
    • Qip2 antibody
    • SRP1 antibody
    • SRP1-gamma antibody
    • SRP1gamma antibody
    • SRP4 antibody
    see all

Anti-KPNA3 antibody images

  • Immunohistochemical analysis of KPNA3 expression in Formalin-Fixed, Paraffin-Embedded Human Adrenal using 5 µg/ml ab117578.
  • Immunohistochemical analysis of KPNA3 expression in Formalin-Fixed, Paraffin-Embedded Human Brain, Cerebellum, using 5 µg/ml ab117578.
  • Immunohistochemical analysis of KPNA3 expression in Formalin-Fixed, Paraffin-Embedded Human Colon using 5 µg/ml ab117578.

References for Anti-KPNA3 antibody (ab117578)

ab117578 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab117578.
Please use the links above to contact us or submit feedback about this product.