The nuclear import of karyophilic proteins is directed by short amino acid sequences termed nuclear localization signals (NLSs). Karyopherins, or importins, are cytoplasmic proteins that recognize NLSs and dock NLS-containing proteins to the nuclear pore complex. The protein encoded by this gene shares the sequence similarity with Xenopus importin-alpha and Saccharomyces cerevisiae Srp1. This protein is found to interact with the NLSs of DNA helicase Q1 and SV40 T antigen.
Our Abpromise guarantee covers the use of ab6039 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 1 - 2 µg/ml.|
|WB||Use a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 55-60 kDa (predicted molecular weight: 58 kDa).Can be blocked with Human KPNA4 peptide (ab23144). A customer reported that milk can significantly suppress antigen-antibody interaction on nitrocellulose membrane in WB.|
Immunohistochemical analysis of 10% buffered formalin-fixed paraffin-embedded murine nephric tissue sections, labelling KPNA4 with ab6039 at a dilution of 1/100 incubated for 12 hours at 4°C. Heat mediated antigen retrival was performed through 10mM citrate buffer at pH 6.0. Blocking was with 5% serum incubated for 1 hour at 21°C. Secondary was a polyclonal Donkey anti-Goat Alexa Fluor® 647 conjugate at 1/200. Counterstain is DAPI against nuclear DNA. The Negative control is the same as above, but in the abscence of the primary ab6039.