Anti-KPNA5 antibody - N-terminal (ab189987)

Overview

  • Product name
    Anti-KPNA5 antibody - N-terminal
    See all KPNA5 primary antibodies
  • Description
    Rabbit polyclonal to KPNA5 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human KPNA5 (N terminal). The exact sequence is proprietary. (18 amino acid peptide near the amino terminus).
    Database link: O15131

  • Positive control
    • Human brain cortex tissue; EL4 cells; EL4 cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab189987 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 58 kDa.
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 5 - 20 µg/ml.

Target

  • Function
    Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates nuclear import of STAT1 homodimers and STAT1/STAT2 heterodimers by recognizing non-classical NLSs of STAT1 and STAT2 through ARM repeats 8-9. Recognizes influenza A virus nucleoprotein through ARM repeat 7-9 In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS.
  • Tissue specificity
    Testis.
  • Sequence similarities
    Belongs to the importin alpha family.
    Contains 10 ARM repeats.
    Contains 1 IBB domain.
  • Domain
    Consists of an N-terminal hydrophilic region, a hydrophobic central region composed of 10 repeats, and a short hydrophilic C-terminus. The N-terminal hydrophilic region contains the importin beta binding domain (IBB domain), which is sufficient for binding importin beta and essential for nuclear protein import.
    The IBB domain is thought to act as an intrasteric autoregulatory sequence by interacting with the internal autoinhibitory NLS. Binding of KPNB1 probably overlaps the internal NLS and contributes to a high affinity for cytoplasmic NLS-containing cargo substrates. After dissociation of the importin/substrate complex in the nucleus the internal autohibitory NLS contributes to a low affinity for nuclear NLS-containing proteins.
    The major and minor NLS binding sites are mainly involved in recognition of simple or bipartite NLS motifs. Structurally located within in a helical surface groove they contain several conserved Trp and Asn residues of the corresponding third helices (H3) of ARM repeats which mainly contribute to binding.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • IMA5_HUMAN antibody
    • Importin alpha 6 antibody
    • Importin subunit alpha 6 antibody
    • Importin subunit alpha-6 antibody
    • IPOA 6 antibody
    • IPOA6 antibody
    • Karyopherin alpha 5 antibody
    • Karyopherin alpha 5 importin alpha 6 antibody
    • Karyopherin subunit alpha 5 antibody
    • Karyopherin subunit alpha-5 antibody
    • KPNA 5 antibody
    • KPNA5 antibody
    • SRP 6 antibody
    • SRP6 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain cortex tissue labeling KPNA5 with ab189987 at 5 µg/ml.

  • Immunofluorescent analysis of EL4 cells labeling KPNA5 with ab189987 at 20 µg/ml.

  • Immunocytochemical analysis of EL4 cells labeling KPNA5 with ab189987 at 5 µg/ml.

  • All lanes : Anti-KPNA5 antibody - N-terminal (ab189987) at 1 µg/ml

    Lane 1 : EL4 cell lysate
    Lane 2 : EL4 cell lysate with immunizing peptide

    Predicted band size: 58 kDa

References

ab189987 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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