• Product nameAnti-KRAS+HRAS+NRAS antibody
    See all KRAS+HRAS+NRAS primary antibodies
  • Description
    Mouse monoclonal to KRAS+HRAS+NRAS
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment (GST-tag) corresponding to Human KRAS+HRAS+NRAS aa 16-125.


    Database link: P01116



Our Abpromise guarantee covers the use of ab55391 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 22 kDa.
IHC-P Use at an assay dependent concentration.
Flow Cyt Use 0.5-1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.


  • RelevanceRas proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the regulation of cell proliferation. Enzyme regulation: Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP). Interaction with SOS1 promotes exchange of bound GDP by GTP.
  • Cellular localizationCell Membrane, Cytoplasmic and Golgi Apparatus
  • Database links
  • Alternative names
    • c-H-ras antibody
    • c-K-ras antibody
    • c-Ki-ras antibody
    • GTPase HRas antibody
    • GTPase KRas antibody
    • GTPase NRas antibody
    • H-Ras-1 antibody
    • Ha-Ras antibody
    • HRAS1 antibody
    • K-Ras 2 antibody
    • Ki-Ras antibody
    • KRAS antibody
    • KRAS2 antibody
    • NRAS antibody
    • p21ras antibody
    • RASK2 antibody
    • Transforming protein N-Ras antibody
    • Transforming protein p21 antibody
    see all

Anti-KRAS+HRAS+NRAS antibody images

  • Predicted band size : 22 kDa
    cK Ras antibody (ab55391) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • Overlay histogram showing HeLa cells stained with ab55391 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55391, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a slightly decreased signal in HeLa cells fixed with methanol (5 min)/permeabilized with 0.1% PBS-Tween 20 used under the same conditions.

  • ab55391 staining KRAS+HRAS+NRAS in Human Gastric Acenocarcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% phosphate buffered formalin, permeabilized with 0.025% of triton X -100 in TBS and blocked with 3% hydrogen peroxide for 5 minutes at room temperature; antigen retrieval was by heat mediation in target retrieval solution. Samples were incubated with primary antibody (1/100 in 0.025% triton X-100-TBS) for 1 hour. An HRP polymer was used for detection.

References for Anti-KRAS+HRAS+NRAS antibody (ab55391)

This product has been referenced in:
  • Bäumer S  et al. Antibody-mediated delivery of anti-KRAS-siRNA in vivo overcomes therapy resistance in colon cancer. Clin Cancer Res 21:1383-94 (2015). WB . Read more (PubMed: 25589625) »
  • Salloum D  et al. Mutant ras elevates dependence on serum lipids and creates a synthetic lethality for rapamycin. Mol Cancer Ther 13:733-41 (2014). Read more (PubMed: 24435447) »

See all 8 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Gel Running Conditions Reduced Denaturing (12.5%)
Loading amount 1e+006 cells
Specification HeLa
Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Jan 12 2016

The immunogen for this antibody is KRAS (NP_004976, 16 a.a. ~ 126 a.a). As we don't do epitope mapping, we can't provide you the exactly epitope where the antibody is targeting on. Therefore without testing, we cannot be sure if the mutated form of KRA...

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Thank you for contacting Abcam earlier today.

I am sorry that you were having some issues with ab55391. The antibody is covered under our Abpromise for six months and is guaranteed to work in IHC-P on human samples . Based on the protocol info...

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Merci pour ces précisions.

Comme convenu j'ai mis en place l'envoi d'une unité d'anti-KRAS ab55391. Le numéro de commande de remplacement gratuit de ce produit est *****. Vous recevrez prochainement un mail de confirmati...

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Thank you for contacting us.

The current in stock lots are GR47401-1, GR47401-2.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for contacting Abcam.

Under our Abpromise we guarantee that all of those antibodies you listed would work on human tissue in IHC-P. However I would recommend ab55391, as It has protocol information available on the website.
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Merci d'avoir pris le temps de remplir notre questionnaire et de nous avoir contactés. Je suis désolé d'apprendre que vous avez eu des difficultés à obtenir de bons résultats avec ab84573.

Notre poli...

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Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibodyhas not been providing satisfactory results despite the protocol tips provided.

The details provided will enable us to investigate...

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Thank you for clarifying the situation. Having reviewed the results and the protocol used I would recommend the following:

1.) Although I have found reference of KRAS expression in some of the used cell lines. I would still suggest usin...

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Thank you for taking time to liaising with the customer and to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results. Having reviewed this case, I would like to offer some suggestions to help optimize t...

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