Overview

  • Product name
  • Description
    Rabbit polyclonal to KRI1
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Recombinant fragment, corresponding to amino acids 360-468 of Human KRI1

  • Positive control
    • RT-4, U-251 MG, Human Plasma, Human Liver and Human Tonsil cell lysates, Human cerebral cortex tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 50% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab150908 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250 - 1/500. Predicted molecular weight: 83 kDa.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

Images

  • Immunofluorescent staining of Human cell line MCF-7 shows positivity in nucleoli. Recommended concentration of ab150908 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-KRI1 antibody (ab150908) at 1/250 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human Plasma cell lysate
    Lane 4 : Human Liver cell lysate
    Lane 5 : Human Tonsil cell lysate


    Predicted band size : 83 kDa
  • Immunohistochemical analysis of paraffin embedded Human cerebral cortex tissue labeling KRI1 with ab150908 antibody at 1/500.

References

ab150908 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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